140
R.T. Vinarao et al.
Table 12.1 Participating stations and their geographical location,
May 2009 and April 2010
Sampling area
Station No. Adjacent municipality
Geographic location
1
Raois, La Union
16
ı 16
0 46N; 120
ı 22
0 22E
2
Cayanga, San Fabian
16
ı 06
0 73N; 120
ı 23
0 83E
3
Sobol, San Fabian
16
ı 07
0 89N; 120
ı 24
0 45E
4
Binloc, Dagupan
16
ı 06
0 25N; 120
ı 22
0 60E
5
Lucao, Dagupan
16
ı 17
0 52N; 119
ı 54
0 02E
6
Manat, Binmaley
16
ı 00
0 88N; 120
ı 17
0 15E
7
Sagur, Labrador
16
ı 03
0 08N; 120
ı 07
0 07E
8
San Jose, Labrador
16
ı 02
0 00N; 120
ı 08
0 60E
9
Centro, Sual
16
ı 03
0 54N; 120
ı 05
0 52E
10
Mona, Alaminos
16
ı 13
0 11N; 119
ı 58
0 56E
11
Tambac, Bani
16
ı 16
0 00N; 119
ı 55
0 33E
12
Anda-Bolinao Boundary 16
ı 17
0 52N; 119
ı 54
0 02E
Sample Collection
The first sampling, representing the monsoon season, was conducted in May 2009,
while the second, representing the pre-monsoon season, was completed in April
2010, prior to the onset of heavy rains.
Oyster Tissue
Cultivated oysters were handpicked (gloved hands) directly from the oyster growing
strings and stake poles with the aid of knife. Samples were transported and preserved
following the method described by BFAR (2001a). Twenty-five to thirty (25–30)
pieces of shelled oysters, with nearly equal size per station, were packed in a
26.8 27.3 cm Ziploc
TM plastic bag and transported in an icebox to the laboratory
for cleaning and shucking. Samples were brushed and initially rinsed with seawater
(collected at the sampling site) until all attached particulate matter was removed,
then finally with distilled water. The cleaned oysters were then shucked using
shucking knives. Two hundred grams of soft tissue from the shucked oysters were
double-packed (inner stomacher bag; outer Ziploc
TM bag) and preserved at 4
ı C
temperature until analyzed.
Sediment
Two hundred grams (200 g) of sediment samples were collected at the shallow
waters near the banks of the sampling stations using a samples. All samples were
taken from the top layer to a depth of over 30 cm, at stations where the flow rates
R.T. Vinarao et al.
Table 12.1 Participating stations and their geographical location,
May 2009 and April 2010
Sampling area
Station No. Adjacent municipality
Geographic location
1
Raois, La Union
16
ı 16
0 46N; 120
ı 22
0 22E
2
Cayanga, San Fabian
16
ı 06
0 73N; 120
ı 23
0 83E
3
Sobol, San Fabian
16
ı 07
0 89N; 120
ı 24
0 45E
4
Binloc, Dagupan
16
ı 06
0 25N; 120
ı 22
0 60E
5
Lucao, Dagupan
16
ı 17
0 52N; 119
ı 54
0 02E
6
Manat, Binmaley
16
ı 00
0 88N; 120
ı 17
0 15E
7
Sagur, Labrador
16
ı 03
0 08N; 120
ı 07
0 07E
8
San Jose, Labrador
16
ı 02
0 00N; 120
ı 08
0 60E
9
Centro, Sual
16
ı 03
0 54N; 120
ı 05
0 52E
10
Mona, Alaminos
16
ı 13
0 11N; 119
ı 58
0 56E
11
Tambac, Bani
16
ı 16
0 00N; 119
ı 55
0 33E
12
Anda-Bolinao Boundary 16
ı 17
0 52N; 119
ı 54
0 02E
Sample Collection
The first sampling, representing the monsoon season, was conducted in May 2009,
while the second, representing the pre-monsoon season, was completed in April
2010, prior to the onset of heavy rains.
Oyster Tissue
Cultivated oysters were handpicked (gloved hands) directly from the oyster growing
strings and stake poles with the aid of knife. Samples were transported and preserved
following the method described by BFAR (2001a). Twenty-five to thirty (25–30)
pieces of shelled oysters, with nearly equal size per station, were packed in a
26.8 27.3 cm Ziploc
TM plastic bag and transported in an icebox to the laboratory
for cleaning and shucking. Samples were brushed and initially rinsed with seawater
(collected at the sampling site) until all attached particulate matter was removed,
then finally with distilled water. The cleaned oysters were then shucked using
shucking knives. Two hundred grams of soft tissue from the shucked oysters were
double-packed (inner stomacher bag; outer Ziploc
TM bag) and preserved at 4
ı C
temperature until analyzed.
Sediment
Two hundred grams (200 g) of sediment samples were collected at the shallow
waters near the banks of the sampling stations using a samples. All samples were
taken from the top layer to a depth of over 30 cm, at stations where the flow rates