58
Methods for Setting Up Primary Cultures Specific to Animal Groups
Sterilization
and
dissection
Culture
set-up
• • Materials
The materials used are the same as those given for sub protocol 1.
• • Procedure
1. Follow the instructions given for sterilization, and steps 1-4 for dissection as listed in subprotocol 1.
2. Take out the heart with the surrounding tissues.
1. Clean the heart by removing as much unnecessary tissue as possible,
but leaving the aorta, alary muscles and pericardial cells attached to
the heart.
2. Rinse the tissues in physiological solution, and transfer them into culture medium.
Fig. 9. Heart system of the cabbage armyworm,
Mamestra brassicae. F, fat bodies; H, heart (dorsal
vessel); T, tracheal system. The larva was cut open
from the ventral side, and the alimentary tract
was removed. xO.9
Fig. 10. Cell migration from
heart tissue of the cabbage armyworm Mamestra brassicae. x
100
Methods for Setting Up Primary Cultures Specific to Animal Groups
Sterilization
and
dissection
Culture
set-up
• • Materials
The materials used are the same as those given for sub protocol 1.
• • Procedure
1. Follow the instructions given for sterilization, and steps 1-4 for dissection as listed in subprotocol 1.
2. Take out the heart with the surrounding tissues.
1. Clean the heart by removing as much unnecessary tissue as possible,
but leaving the aorta, alary muscles and pericardial cells attached to
the heart.
2. Rinse the tissues in physiological solution, and transfer them into culture medium.
Fig. 9. Heart system of the cabbage armyworm,
Mamestra brassicae. F, fat bodies; H, heart (dorsal
vessel); T, tracheal system. The larva was cut open
from the ventral side, and the alimentary tract
was removed. xO.9
Fig. 10. Cell migration from
heart tissue of the cabbage armyworm Mamestra brassicae. x
100