42 Large-Scale Cell Culture
389
• • Comments
If the cells are attached to a micro carrier, such as glass beads or plastic
beads, various culture systems may be used. Shuler et al. (1989) constructed a packed-bed reactor (Fig. 2) for culturing Trichoplusia ni cells
(Tn SBI-4, a variant of the TN-368 cell line showing strong adhesiveness). These authors used non-porous glass beads with a 3 mm diameter. The gas-exchange system consisted of an air-lift pump, and was separated from the cell column. The oxygenated medium recirculated through
the packed-bed reactor. A sufficient oxygen supply was achieved by a
fluid recirculation rate of 90 ml/h. In the cell column, cells grew to a monolayer on the upper halves of the beads, because the reactor was inoculated from the top. The cells grew with a doubling time of 16 h.
Agathos et al. (1989) cultured Aedes albopictus cells with a modified
Eagle's MEM in a spinner reactor. For supporting anchorage-dependent
~Ir t Ut u
Fo.rrut\9 fr' D
..
Alrlltt
At r F lOwtntltr
Fig. 2. Schematic diagram of the packed-bed insect cell bioreactor and air-lift for
oxygen supplementation. From Shuler, M.L. et. al. (1989), reproduced with permission of New York Academy of Sciences
Fig. 3. A spinner culture system. The upper part is the
spinner flask, while the lower part is the magnetic stirrer
389
• • Comments
If the cells are attached to a micro carrier, such as glass beads or plastic
beads, various culture systems may be used. Shuler et al. (1989) constructed a packed-bed reactor (Fig. 2) for culturing Trichoplusia ni cells
(Tn SBI-4, a variant of the TN-368 cell line showing strong adhesiveness). These authors used non-porous glass beads with a 3 mm diameter. The gas-exchange system consisted of an air-lift pump, and was separated from the cell column. The oxygenated medium recirculated through
the packed-bed reactor. A sufficient oxygen supply was achieved by a
fluid recirculation rate of 90 ml/h. In the cell column, cells grew to a monolayer on the upper halves of the beads, because the reactor was inoculated from the top. The cells grew with a doubling time of 16 h.
Agathos et al. (1989) cultured Aedes albopictus cells with a modified
Eagle's MEM in a spinner reactor. For supporting anchorage-dependent
~Ir t Ut u
Fo.rrut\9 fr' D
..
Alrlltt
At r F lOwtntltr
Fig. 2. Schematic diagram of the packed-bed insect cell bioreactor and air-lift for
oxygen supplementation. From Shuler, M.L. et. al. (1989), reproduced with permission of New York Academy of Sciences
Fig. 3. A spinner culture system. The upper part is the
spinner flask, while the lower part is the magnetic stirrer