Chapter 30
Porifera
Subprotocol 1
Ciliated Layer of Sponge Larva (Demospongia)
Materials
- Moist chamber
- Slide glasses
- Petri dishes
- Pasteur pipettes
- Seawater
- Sponge Mycale contarenii
Procedure
1. Excise cell groups from the ciliated layer of a sponge larva.
2. Place the excised cell groups on a glass slide in a drop of filtered seawater containing penicillin (1,000 IU/ml) and streptomycin (0.25 mgl
ml).
1. Keep the slide in a moist chamber with 100 % relative humidity.
2. Maintain the culture at Iso-20°e.
3. Transfer the tissues into fresh medium every 2 days.
Results
According to Borojevic (1966), the archaeocytes that were contained in
the central part of the larva differentiated progressively into choanoblasts
and choanocytes. A functional sponge was obtained 3-4 days after the
beginning of culture. The larval ciliate epithelium transformed rapidly
into a great number of normal flagellate chambers. The archaeocytes
were able to differentiate into both choanocytes and collencytes.
References
PROTOCOL
Equipment
Solutions
Animals
Culture
set-up
Maintenance
of cultures
Borojevic, R. (1966) Etude experimentale de la differenciation des cellules de l'eponge au cours de
son developpement. Dev. Bioi. 14, 130-153.
Porifera
Subprotocol 1
Ciliated Layer of Sponge Larva (Demospongia)
Materials
- Moist chamber
- Slide glasses
- Petri dishes
- Pasteur pipettes
- Seawater
- Sponge Mycale contarenii
Procedure
1. Excise cell groups from the ciliated layer of a sponge larva.
2. Place the excised cell groups on a glass slide in a drop of filtered seawater containing penicillin (1,000 IU/ml) and streptomycin (0.25 mgl
ml).
1. Keep the slide in a moist chamber with 100 % relative humidity.
2. Maintain the culture at Iso-20°e.
3. Transfer the tissues into fresh medium every 2 days.
Results
According to Borojevic (1966), the archaeocytes that were contained in
the central part of the larva differentiated progressively into choanoblasts
and choanocytes. A functional sponge was obtained 3-4 days after the
beginning of culture. The larval ciliate epithelium transformed rapidly
into a great number of normal flagellate chambers. The archaeocytes
were able to differentiate into both choanocytes and collencytes.
References
PROTOCOL
Equipment
Solutions
Animals
Culture
set-up
Maintenance
of cultures
Borojevic, R. (1966) Etude experimentale de la differenciation des cellules de l'eponge au cours de
son developpement. Dev. Bioi. 14, 130-153.