14
General Methods
1
2
3 4
5
6
Fig. 24. Dissecting tools. 1, an iris knife; 2, a surgical knife; 3,4, watchmakers' forceps; 5,6, ophthalmologists' scissors
Fig. 25. Very fine insect pins
(arrows)
Fig. 26. Various-sized plastic culture
flasks. Upper left 250 ml, 75 em';
upper right 250 ml, 75 em'; lower left
25 ml, 12.5 em'; lower middle 50 ml,
25 em'; lower right 50 ml, 25 em'
petri dishes, culture tubes, T-flasks, Carrel flasks, Leighton tubes, twin
tubes, Labtec chambers, and so on. It is important that the inner surface
of the vessel be smooth. In order to get fine images under a phase-contrast microscope, the thickness of the vessel wall should be uniform, and
any scratches or air bubbles should be avoided. Both glass and plastic
vessels are available. Glassware can be reused by repeated washing and
sterilization between use. Most plastic ware is made from polystyrene.
The inner surface of some plastic vessels can be coated with substances
such as collagen, poly L-Iysine, fibronectin, or vitronectin to enhance cellular adhesiveness. These vessels are used mostly for closed and stationary cultures. In order to circulate the culture medium during culture, a
Rose culture chamber (for example Ikemoto Rika) is used. For the largescale culture of suspended cells, a spinner bottle and a magnetic stirrer
are commonly used. However, suspended cells in media may be cultured
in an Erlenmeyer flask by placing the flask on a rotary shaker or a reciprocate shaker. For the culture of substrate-dependent cells, roller bottles
are used. For details of large-scale cell culture at the industrial level, refer to chapter 42, Large-scale Cell Culture.
General Methods
1
2
3 4
5
6
Fig. 24. Dissecting tools. 1, an iris knife; 2, a surgical knife; 3,4, watchmakers' forceps; 5,6, ophthalmologists' scissors
Fig. 25. Very fine insect pins
(arrows)
Fig. 26. Various-sized plastic culture
flasks. Upper left 250 ml, 75 em';
upper right 250 ml, 75 em'; lower left
25 ml, 12.5 em'; lower middle 50 ml,
25 em'; lower right 50 ml, 25 em'
petri dishes, culture tubes, T-flasks, Carrel flasks, Leighton tubes, twin
tubes, Labtec chambers, and so on. It is important that the inner surface
of the vessel be smooth. In order to get fine images under a phase-contrast microscope, the thickness of the vessel wall should be uniform, and
any scratches or air bubbles should be avoided. Both glass and plastic
vessels are available. Glassware can be reused by repeated washing and
sterilization between use. Most plastic ware is made from polystyrene.
The inner surface of some plastic vessels can be coated with substances
such as collagen, poly L-Iysine, fibronectin, or vitronectin to enhance cellular adhesiveness. These vessels are used mostly for closed and stationary cultures. In order to circulate the culture medium during culture, a
Rose culture chamber (for example Ikemoto Rika) is used. For the largescale culture of suspended cells, a spinner bottle and a magnetic stirrer
are commonly used. However, suspended cells in media may be cultured
in an Erlenmeyer flask by placing the flask on a rotary shaker or a reciprocate shaker. For the culture of substrate-dependent cells, roller bottles
are used. For details of large-scale cell culture at the industrial level, refer to chapter 42, Large-scale Cell Culture.