28 Platyhelminthes
297
2. Wash the fragments in several changes of sterile Ringer's solution and
finally in Gey's solution.
1. Transfer the fragments into a roller tube containing culture medium. Culture
2. Culture at 38°C.
set-up
Results
According to Schiller et al. (1959), a 30-fold increase in size of the cultured fragments was observed by the 11th day of culture if the medium
was supplemented with an extract of cestode tissue. The scolex regenerated strobila. As many as 130 segments formed, with some differentiation of reproductive organs. When the cultured worm was implanted in
rats, it became a single, normal H. diminuta.
References
Betchaku, T. (1970) The cellular mechanism of the formation of a regeneration blastema of freshwater Planaria, Dugesia dorotocephala. 1. The behavior of cells in a tiny body fragment isolated in vitro. J. Exp. Zool. 174,253-280.
Sengel, C. (1960) Culture in vitro de blastemes de regeneration de Planaires. J. Embryol. Exp. Morphol.
8,468-476.
Schiller, E.L., Read, c.P. and Rothman, A.H. (1959) Preliminary experiments on the growth of a
cyclophyllidean cestode in vitro. J. Parasitol. 45 (Suppl.), 29. (Abstract)
Stachowitsch, M. (1992) The Invertebrates, an illustrated glossary. Wiley-Liss, New York, pp676.
297
2. Wash the fragments in several changes of sterile Ringer's solution and
finally in Gey's solution.
1. Transfer the fragments into a roller tube containing culture medium. Culture
2. Culture at 38°C.
set-up
Results
According to Schiller et al. (1959), a 30-fold increase in size of the cultured fragments was observed by the 11th day of culture if the medium
was supplemented with an extract of cestode tissue. The scolex regenerated strobila. As many as 130 segments formed, with some differentiation of reproductive organs. When the cultured worm was implanted in
rats, it became a single, normal H. diminuta.
References
Betchaku, T. (1970) The cellular mechanism of the formation of a regeneration blastema of freshwater Planaria, Dugesia dorotocephala. 1. The behavior of cells in a tiny body fragment isolated in vitro. J. Exp. Zool. 174,253-280.
Sengel, C. (1960) Culture in vitro de blastemes de regeneration de Planaires. J. Embryol. Exp. Morphol.
8,468-476.
Schiller, E.L., Read, c.P. and Rothman, A.H. (1959) Preliminary experiments on the growth of a
cyclophyllidean cestode in vitro. J. Parasitol. 45 (Suppl.), 29. (Abstract)
Stachowitsch, M. (1992) The Invertebrates, an illustrated glossary. Wiley-Liss, New York, pp676.