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I. Excise the organs under aseptic conditions.
I. Place the organs in culture medium.
2. Maintain the culture at 20°e.
Results
According to Malecha (1965), ovaries survived for 20 days without noticeable structural changes, but no maturation of ovocytes was observed.
The epithelium of the oviduct also survived for 13 days. When the integument was cultured with attached muscle, the cut surface was healed
within 3 days, and the tissues survived more than 2 months. Malecha
(1967a) also reported that spermatogonia decreased in number, and spermatocytes disappeared during a 2-week culture of testes. However, when
nervous systems around the pharynx were co cultured with testes, spermatogenesis proceeded, and spermatozoids were formed.
Subprotocol 4
Para podia of Nereids (Polychaeta)
Materials
- The equipment used is the same as given for subprotocoll
- Durchon and Schaller's medium (see Appendix 1)
- Nereis pelagica
- Platynereis dumerilii
- Perinereis cultrifera
- Nereis diversicolor
Procedure
Dissection
Culture
set-up
Equipment
Solutions
Animals
1. Keep the animals in sterile seawater for 3-4 days before dissection.
Sterilization
2. Surface sterilize the animals by irradiation with a UV ray for 10 min
in a laminar flow cabinet.
1. Dissect out parapodia (Fig. 1).
Dissection
1. Place the excised organs in culture medium.
Culture
2. Maintain the culture at 20°e.
set-up
3. Transfer the organs to fresh medium every 4 days.