25 Mollusca
285
Sperm duct
Vas deferens
Seminal
vesicle
Sperm bulb
Oviducal
opening
Oviduct
~'H:c~ Nldamental
glands
Fig.!. Reproductive systems of
male (left) and female (right)
squid
1. Place the organs in a culture vessel with culture medium.
2. Keep the culture at 21 °C under darkness.
• • Results
According to Durchon and Richard (1967), the optic glands of 4-6-monthold cuttlefish showed the secretion process as early as 4th day in culture.
In cultures of immature ovaries cultured alone, the ovocytes continued
their growth, but the germinative cord showed no resumption of activity. When ovaries and optic gland were cultured together, ovocytes larger
than 0.3 mm doubled their size in 20 days.
• References
Bailey, T.G. (1969) Anaesthetic technique for slugs. Experientia 25, 1225.
Culture
set-up
Durchon, M. and Richard, A. (1967) Etude, en culture organotypique, du role endocrine de la glande
optique dans la maturation ovarienne chez Sepia officinalis L. (Mollusque Cephalopode). C.
R. Acad. Sci. 264, 1497-1500.
Wattez, C. (1978) Influence du complexe cephalique (tentacles oculaires-cerveau) sur i'evolution,
en culture in vitro, de gonades infantiles et juveniles d'Arion subfuscus Drap. (Gasteropode
Pulmone). Gen. Compo Endocrinol. 35, 360-374.
285
Sperm duct
Vas deferens
Seminal
vesicle
Sperm bulb
Oviducal
opening
Oviduct
~'H:c~ Nldamental
glands
Fig.!. Reproductive systems of
male (left) and female (right)
squid
1. Place the organs in a culture vessel with culture medium.
2. Keep the culture at 21 °C under darkness.
• • Results
According to Durchon and Richard (1967), the optic glands of 4-6-monthold cuttlefish showed the secretion process as early as 4th day in culture.
In cultures of immature ovaries cultured alone, the ovocytes continued
their growth, but the germinative cord showed no resumption of activity. When ovaries and optic gland were cultured together, ovocytes larger
than 0.3 mm doubled their size in 20 days.
• References
Bailey, T.G. (1969) Anaesthetic technique for slugs. Experientia 25, 1225.
Culture
set-up
Durchon, M. and Richard, A. (1967) Etude, en culture organotypique, du role endocrine de la glande
optique dans la maturation ovarienne chez Sepia officinalis L. (Mollusque Cephalopode). C.
R. Acad. Sci. 264, 1497-1500.
Wattez, C. (1978) Influence du complexe cephalique (tentacles oculaires-cerveau) sur i'evolution,
en culture in vitro, de gonades infantiles et juveniles d'Arion subfuscus Drap. (Gasteropode
Pulmone). Gen. Compo Endocrinol. 35, 360-374.