22 Arthropoda Other than Insecta
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1. Place the explants on lens paper floating on culture medium, which Culture
has been placed in a watchglass.
set-up
2. Place the watchglass in a petri dish carpeted with a ring of wet cotton
wool, and incubate the culture 25°C,
Results
According to the 1 week culture performed by Oyama and Kamemoto
(1970), the young developing oocytes could be maintained and the germinative zone with numerous cell divisions was not affected. The culture medium promoted the proliferation of interstitial cells, and some
growth of young oocytes. However, maturing oocytes in later stages of
development were not maintained.
Subprotocol 8
Dorsal Vessels, Nervous Systems, Pancreases, Gonads, and
Intestines of Limulus (Merostomacea)
Wolff (1962) cultured various organs of Limulus.
Materials
- Dissecting tray
- Scissors
- Forceps
- Watch glasses
- Culture vessels
- Sterilized seawater
- Culture medium (see Results)
- Limulus, Xiphosura polyphemus
Procedure
Equipment
Solutions
Animals
1. Wash the animals many times with seawater sterilized by filtration.
Sterilization
2. Keep the animals in sterilized seawater containing penicillin (150 lUI
ml) for several hours.
1. Dissect the animals as soon as possible after sterilization.
Dissection
2. Keep the excised organs in watchglasses filled with sterilized seawater
containing penicillin (150 IU/ml).
1. Wash the organs many times with fresh sterile seawater containing Culture
penicillin.
set-up