21 Insecta
259
1. Dissect out the testes in a dissecting tray filled with physiological so- Dissection
lution (Fig. 7).
1. Clean the testes of attached tissues in a Maximov slide, the depression Culture
of which is filled with physiological solution.
set-up
2. Transfer the testes into a culture vessel with culture media.
Results
According to Yagi et al. (1969), the testes of the rice stem borer C.
suppressalis were able to be maintained in CSM-2F medium (see Appendix 1) for more than 1 month in healthy condition. During culture, the
testes increased in volume, and spermatogenesis proceeded markedly
when 20-hydroxyecdysone was added to the culture medium (Fig. 8).
When isolated spermatocysts were cultured, the cysts elongated and spermiogenesis was observed. A similar result was obtained in the culture of
testes of the greater wax moth G. mellonella (Lender and Duveau -Hagege,
1962; Mano and Mitsuhashi, 1981).
In the cultivation of testes of insects other than Lepidoptera, testes of the
grasshopper Chorthippus curtipennis (Lewis, 1916) and the blowfly C.
erythrocephala (Leloup, 1969) have been reported to show spermiogenesis in vitro.
Subprotocol 16
Ovaries of Various Insects
Materials
- Dissecting microscope and dissecting tray (see chapter 1, section 3 Equipment
"Tools")
- Scissors
- Forceps
- Needles
- Pasteur pipettes
- Culture vessels
- Petri dishes
- Laverdure's physiological solution (see Appendix 1)
- Culture medium (Laverdure's medium; see Appendix 1)
- Larvae of the meal worm Tenebrio molitor
Procedure
The procedures for sterilization, dissection and culture are the same as
those given for the preparation of cultures of testes from lepidopteran
insects.
Solutions
Animals
259
1. Dissect out the testes in a dissecting tray filled with physiological so- Dissection
lution (Fig. 7).
1. Clean the testes of attached tissues in a Maximov slide, the depression Culture
of which is filled with physiological solution.
set-up
2. Transfer the testes into a culture vessel with culture media.
Results
According to Yagi et al. (1969), the testes of the rice stem borer C.
suppressalis were able to be maintained in CSM-2F medium (see Appendix 1) for more than 1 month in healthy condition. During culture, the
testes increased in volume, and spermatogenesis proceeded markedly
when 20-hydroxyecdysone was added to the culture medium (Fig. 8).
When isolated spermatocysts were cultured, the cysts elongated and spermiogenesis was observed. A similar result was obtained in the culture of
testes of the greater wax moth G. mellonella (Lender and Duveau -Hagege,
1962; Mano and Mitsuhashi, 1981).
In the cultivation of testes of insects other than Lepidoptera, testes of the
grasshopper Chorthippus curtipennis (Lewis, 1916) and the blowfly C.
erythrocephala (Leloup, 1969) have been reported to show spermiogenesis in vitro.
Subprotocol 16
Ovaries of Various Insects
Materials
- Dissecting microscope and dissecting tray (see chapter 1, section 3 Equipment
"Tools")
- Scissors
- Forceps
- Needles
- Pasteur pipettes
- Culture vessels
- Petri dishes
- Laverdure's physiological solution (see Appendix 1)
- Culture medium (Laverdure's medium; see Appendix 1)
- Larvae of the meal worm Tenebrio molitor
Procedure
The procedures for sterilization, dissection and culture are the same as
those given for the preparation of cultures of testes from lepidopteran
insects.
Solutions
Animals