258
Organ Culture
- Maximov slide
- Culture vessel
Solutions - Physiological solution
- Culture media
Animals - Larvae or pupae of lepidopteran insects
• • Procedure
Sterilization 1. Surface sterilize the animals by submersion in 70% ethanol.
2. Wash the animals in sterilized distilled water.
B
Fig. 7. Testes of the last instar larva of the rice
stem borer Chilo suppressalis. The testes are
located at the fifth abdominal segment
J ~
I" ~ "·
. ....
I
IIF ' . . r' ..•••. w,; ..
J '
. '\' ,
, •. . .
. .
Fig. 8. Testes of larvae of the rice stem borer Chilo suppressalis cultured for 4 days in
medium without (A) or with (B) 20-hydroxy ecdysone. Reproduced from Yagi, S. et
al. (1969) with permission of Japanese Society of Applied Entomology and Zoology
Organ Culture
- Maximov slide
- Culture vessel
Solutions - Physiological solution
- Culture media
Animals - Larvae or pupae of lepidopteran insects
• • Procedure
Sterilization 1. Surface sterilize the animals by submersion in 70% ethanol.
2. Wash the animals in sterilized distilled water.
B
Fig. 7. Testes of the last instar larva of the rice
stem borer Chilo suppressalis. The testes are
located at the fifth abdominal segment
J ~
I" ~ "·
. ....
I
IIF ' . . r' ..•••. w,; ..
J '
. '\' ,
, •. . .
. .
Fig. 8. Testes of larvae of the rice stem borer Chilo suppressalis cultured for 4 days in
medium without (A) or with (B) 20-hydroxy ecdysone. Reproduced from Yagi, S. et
al. (1969) with permission of Japanese Society of Applied Entomology and Zoology