15 Mollusca
193
• • Comments
Burch and Cuadros (1965) cultured gonadal tissues of Helix pomatia.
The cells survived for 60 days, during which cell division and quantitative growth of the cultures were observed.
Subprotocol 18
Octopus (Cephalopoda) White Bodies
• • Materials
- Scissors
- Forceps
- Pasteur pipette
- Petri dish
- Tube
- Rotation drum
Equipment
- Octopus physiological solution (Necco and Martin, 1963; see Appen- Solutions
dix 1)
- Culture medium (TC-199 medium, the osmolarity of which has been
increased from 0.61 to 2.22 freezing point depression by the addition
of 26.833 gil NaCl)
- Young octopus Octopus vulgaris
Optic tract
Optic
lobe ----~.....,
White --~---,
body
Optic
gland
Anterior sali vary
gland
s.allvary
gland
Fig. 26. Inner structure of
the octopus head. From
Matsumoto, A. and Ishii, S.,
"Atlas of Endocrine Organs,
Vertebrates and Invertebrates", pp.248, Fig.27, 1992,
Copyright © 1992 by SpringerVerlag. Reproduced with permission of copyright owner
Fig. 27. White body cells in culture of Octopus vulgaris. x 130. From Necco,A. and Martin, R., 1963
with permission
Animals