15 Mollusca
191
Mucus gland
Dart sac
Vagina
- Culture vessels
- Pasteur pipettes
- 80-~m nylon filter
- Penicillin G
- Streptomycin sulfate
Albumin
gland
- Dispase (Boehringer-Mannheim)
- Medium 199
Fig. 23. Migration of amoebocyte-like cells from midgut
gland tissue of Oncomelania
hupensis hupensis. x 160. From
Iwanaga, Y. et aI., 1985 with permission
Fig. 24. The hermaphroditic
reproductive system of Helix
- Culture medium (Hank's salts supplemented with 0.2 M NaHC0 3 ,
100 IV/ml penicillin G, 100 ~g/ml streptomycin sulfate, and 20% FBS;
osmotic pressure 270 mOsmollkg; pH 8.1)
- 0.1 % Collagenase-dispase
- Adult snail Helix aspersa
• • Procedure
Chemicals
Solutions
Animals
l. Aseptically remove ovotestes from fresh snails (Fig. 24).
Dissection
2. Wash the gonads with five successive immersions (5 min each) in
PBS containing penicillin G (250 IV/mI) and streptomycin sulfate
(250 ~g/ml).