Mouth
Cerebral ganglion
Adductor muscle
Esophagus
Right scutum
Rostral sinus
12 Arthropods Other than Insects
139
Right tergum
Exopod
}
Endopod
C\g~~
Protopod
Carina
L~~~,---jl'tl\-tt- Anus
Depresspr
muscle
Fig. 14. Vertical section of the acorn barnacle Balanus showing major anatomical features. From "The Invertebrates", Stachowitsch, M., © 1992. Reprinted by permission of
John Wiley & Sons, Inc.
Fig. 15. Cell migration from explants consisting of parenchyma
and ovarioles of the cirripede
Balanus amphitrite. x 400. From
Fyhn, U.E.H. and Costlow, J.D.,
1975 with permission
- Culture medium (0.1 % glucose, 1 % TC bovine embryo extract EE100 Solutions
(Difco Laboratories) in Hoyle and Smyth's (1963) saline, with 100 lUI
ml penicillin and 100 fJg/ml streptomycin)
- Adult barnacles (Balanus amphitrite; 10-15 mm in basal diameter), Animals
Artemia nauplii eggs
- CO 2 gas
Miscellaneous
II. Procedure
1. Brush the barnacles carefully.
Sterilization
2. Keep the barnacles in seawater, with a salinity of 3.0%, in an aerated
aquarium at 23°C for at least 1 week. During this period, feed the barnacles with newly hatched Artemia nauplii every second day.