8 Insecta (Hemiptera)
95
EtOH
Carlson's Soln.
Surrace Slerlllxalion
Ovaries taken out
Fragments 01
Embryos
- ~'" ""r"'" ~
. ... -,,J' '"
•
.... " " ; .. .: .. ' .:.,
"II,. .. ,.
.. - . II • , .
Olspase Soln. Scdlmenlallon
Suspension In
Culture Medium
Fig. 3. Process of embryonic cell culture of aphids. EtOH, ethanol; so/n., solution
Subprotocol 3
Larval Tissues
Fig. 4. Cell migration from the
fragments of embryos of the
aphid Megoura crassicauda. x 80
Growing cells can be obtained from larval tissues. When small larvae of
homopteran insects are used, the explants will simply be torn larvae. In
this case, larvae reared under aseptic conditions should be used. (For
aseptic rearing of leafhoppers, refer to Mitsuhashi and Maramorosch
(1963) or Mitsuhashi (1979)) .
•
<
Materials
The equipment used is the same as given for subprotocoll.
- Physiological solution
- Culture medium
Equipment
Solutions
95
EtOH
Carlson's Soln.
Surrace Slerlllxalion
Ovaries taken out
Fragments 01
Embryos
- ~'" ""r"'" ~
. ... -,,J' '"
•
.... " " ; .. .: .. ' .:.,
"II,. .. ,.
.. - . II • , .
Olspase Soln. Scdlmenlallon
Suspension In
Culture Medium
Fig. 3. Process of embryonic cell culture of aphids. EtOH, ethanol; so/n., solution
Subprotocol 3
Larval Tissues
Fig. 4. Cell migration from the
fragments of embryos of the
aphid Megoura crassicauda. x 80
Growing cells can be obtained from larval tissues. When small larvae of
homopteran insects are used, the explants will simply be torn larvae. In
this case, larvae reared under aseptic conditions should be used. (For
aseptic rearing of leafhoppers, refer to Mitsuhashi and Maramorosch
(1963) or Mitsuhashi (1979)) .
•
<
Materials
The equipment used is the same as given for subprotocoll.
- Physiological solution
- Culture medium
Equipment
Solutions