689
about bacterial fi sh pathogens. Undoubtedly, more modern methods will eventually
enter the realms of fi sh microbiology. These methods may include the development
of highly reliable rapid techniques, such as offered by high-pressure liquid chromatography and mass-spectrometry. Moreover, lipid analyses could be adapted further
for fi sheries work. Serological techniques, such as those involving ELISA and
monoclonal antibodies, are steadily entering the domain of the fi sh disease diagnosticians. In addition, molecular genetic techniques, notably gene probe technology,
are under evaluation in several laboratories.
References
Adams A, Thompson KD, Morris D, Farias C, Chen SC (1995) Development and use of monoclonal antibody probes for immunohistochemistry, ELISA and IFAT to detect bacterial and parasitic fi sh pathogens. Fish Shellfi sh Immunol 5:537–547
Ahne W (1981) Serological techniques currently used in fi sh virology. Dev Biol Stand 49:3–27
Amandi A, Hiu SF, Rohovec JS, Fryer JL (1982) Isolation and characterization of Edwardsiella
tarda from chinook salmon ( Oncorhynchus tshawytscha ). Appl Environ Microbiol
43:1380–1384
Austin B, Allen DA, Zachary A, Belas MR, Colwell RR (1979) Ecology and taxonomy of bacteria
attaching to wood surfaces in a tropical harbor. Can J Microbiol 25:447–461
Austin B, Bishop I, Gray C, Watt B, Dawes J (1986) Monoclonal antibody-based enzyme-linked
immunosorbent assays for the rapid diagnosis of clinical cases of enteric redmouth and furunculosis in fi sh farms. J Fish Dis 9:469–474
Bullock GL, Griffi n BR, Stuckey HM (1980) Detection of Corynebacterium salmoninus by direct
fl uorescent antibody test. Can J Fish Aquat Sci 37:719–721
Busch RA, Lingg A (1975) Establishment of an asymptomatic carrier state infection of enteric
redmouth disease in rainbow trout ( Salmo gairdneri ). J Fish Res Board Can 32:2429–2432
Chang C-I, Hung P-H, Wu C-C, Cheng TC, Tsai J-M, Lin K-J, Lin C-Y (2012) Simultaneous
detection of multiple fi sh pathogens using a naked-eye readable DNA microarray. Sensors
12:2710–2728
Chang W-H, Yang S-Y, Wang C-H, Tsai M-A, Wang P-C, Chen T-Y, Chen S-C, Lee G-B (2013)
Rapid isolation and detection of aquaculture pathogens in an integrated microfl uidic system
using loop-mediated isothermal amplifi cation. Sens Actuators B Chem 180:96–106
Cowan ST (1974) Cowan and steel’s manual for the identifi cation of medical bacteria, 2nd edn.
Cambridge University Press, Cambridge
Del Cerro A, Mendoza MC, Guijarro JA (2002a) Usefulness of a TaqMan-based polymerase chain
reaction assay for the detection of the fi sh pathogen Flavobacterium psychrophilum . J Appl
Microbiol 93:149–156
Del Cerro AL, Marquez I, Guijarro JA (2002b) Simultaneous detection of Aeromonas salmonicida , Flavobacterium psychrophilum and Yersinia ruckeri, three major fi sh pathogens, by multiplex PCR. Appl Environ Microbiol 68:5177–5180
Doetsch RN (1981) 3. Determinative method of light microscopy. In: Gerhardt P (ed) Manual of
methods for general bacteriology. American Society of Microbiology, Washington, DC,
pp 21–23
Eurell TE, Lewis DH, Grumbles LC (1978) Comparison of selected diagnostic tests for detection
of motile Aeromonas septicaemia in fi sh. Am J Vet Res 39:1384–1386
Evensen Ø, Espelid S, Håstein T (1991) Immunohistochemical identifi cation of Vibrio salmonicida in stored tissues of Atlantic salmon Salmosalar from the fi rst known outbreaks of coldwater
vibriosis (‘Hitra disease’). Dis Aquat Org 10:185–189
References
about bacterial fi sh pathogens. Undoubtedly, more modern methods will eventually
enter the realms of fi sh microbiology. These methods may include the development
of highly reliable rapid techniques, such as offered by high-pressure liquid chromatography and mass-spectrometry. Moreover, lipid analyses could be adapted further
for fi sheries work. Serological techniques, such as those involving ELISA and
monoclonal antibodies, are steadily entering the domain of the fi sh disease diagnosticians. In addition, molecular genetic techniques, notably gene probe technology,
are under evaluation in several laboratories.
References
Adams A, Thompson KD, Morris D, Farias C, Chen SC (1995) Development and use of monoclonal antibody probes for immunohistochemistry, ELISA and IFAT to detect bacterial and parasitic fi sh pathogens. Fish Shellfi sh Immunol 5:537–547
Ahne W (1981) Serological techniques currently used in fi sh virology. Dev Biol Stand 49:3–27
Amandi A, Hiu SF, Rohovec JS, Fryer JL (1982) Isolation and characterization of Edwardsiella
tarda from chinook salmon ( Oncorhynchus tshawytscha ). Appl Environ Microbiol
43:1380–1384
Austin B, Allen DA, Zachary A, Belas MR, Colwell RR (1979) Ecology and taxonomy of bacteria
attaching to wood surfaces in a tropical harbor. Can J Microbiol 25:447–461
Austin B, Bishop I, Gray C, Watt B, Dawes J (1986) Monoclonal antibody-based enzyme-linked
immunosorbent assays for the rapid diagnosis of clinical cases of enteric redmouth and furunculosis in fi sh farms. J Fish Dis 9:469–474
Bullock GL, Griffi n BR, Stuckey HM (1980) Detection of Corynebacterium salmoninus by direct
fl uorescent antibody test. Can J Fish Aquat Sci 37:719–721
Busch RA, Lingg A (1975) Establishment of an asymptomatic carrier state infection of enteric
redmouth disease in rainbow trout ( Salmo gairdneri ). J Fish Res Board Can 32:2429–2432
Chang C-I, Hung P-H, Wu C-C, Cheng TC, Tsai J-M, Lin K-J, Lin C-Y (2012) Simultaneous
detection of multiple fi sh pathogens using a naked-eye readable DNA microarray. Sensors
12:2710–2728
Chang W-H, Yang S-Y, Wang C-H, Tsai M-A, Wang P-C, Chen T-Y, Chen S-C, Lee G-B (2013)
Rapid isolation and detection of aquaculture pathogens in an integrated microfl uidic system
using loop-mediated isothermal amplifi cation. Sens Actuators B Chem 180:96–106
Cowan ST (1974) Cowan and steel’s manual for the identifi cation of medical bacteria, 2nd edn.
Cambridge University Press, Cambridge
Del Cerro A, Mendoza MC, Guijarro JA (2002a) Usefulness of a TaqMan-based polymerase chain
reaction assay for the detection of the fi sh pathogen Flavobacterium psychrophilum . J Appl
Microbiol 93:149–156
Del Cerro AL, Marquez I, Guijarro JA (2002b) Simultaneous detection of Aeromonas salmonicida , Flavobacterium psychrophilum and Yersinia ruckeri, three major fi sh pathogens, by multiplex PCR. Appl Environ Microbiol 68:5177–5180
Doetsch RN (1981) 3. Determinative method of light microscopy. In: Gerhardt P (ed) Manual of
methods for general bacteriology. American Society of Microbiology, Washington, DC,
pp 21–23
Eurell TE, Lewis DH, Grumbles LC (1978) Comparison of selected diagnostic tests for detection
of motile Aeromonas septicaemia in fi sh. Am J Vet Res 39:1384–1386
Evensen Ø, Espelid S, Håstein T (1991) Immunohistochemical identifi cation of Vibrio salmonicida in stored tissues of Atlantic salmon Salmosalar from the fi rst known outbreaks of coldwater
vibriosis (‘Hitra disease’). Dis Aquat Org 10:185–189
References