657
0.3 % (w/v) yeast extract
0.5 % (w/v) sodium chloride
1.35 % (w/v) agar
pH 7.0; after boiling to dissolve the ingredients, the medium is not sterilised
further.
Robertsons Meat Broth (= Cooked Meat Medium)
45.4 % (w/v) heart muscle
1 % (w/v) peptone
1 % (w/v) Lab-lemco powder
0.2 % 9 w/v) dextrose
0.5 % (w/v) sodium chloride
pH 7.2
Sterilise at 121 °C/15 min.
Selective Flexibacter Medium (Bullock et al. 1986 )
0.2 % (w/v) tryptone
0.05 % (w/v) yeast extract
0.3 % (w/v) gelatin
1.5 % (w/v) agar
sterilise at 121 °C for 15 min, cool to 45 °C and add fi lter sterilised neomycin sulphate (0.0004 % w/v)
Selective Kidney Disease Medium (SKDM)
1.0 % (w/v) tryptone
0.05 % (w/v) yeast extract
0.005 % (w/v) cycloheximide
1.0 % (w/v) agar
pH 6.8; sterilise at 121 °C for 15 min, cool to 50 °C, add sterile foetal calf serum to
10 % (v/v), and fi lter sterilised solutions of L-cysteine hydrochloride (0.1 % w/v),
D-cycloserine (0.00125 % w/v), polymyxin B sulphate (0.0025 % w/v), and oxolinic acid (0.00025 % w/v).
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens