656
Pseudomonas F Agar
1 % (w/v) tryptone
1 % (w/v) proteose peptone
0.15 % (w/v) dipotassium phosphate
0.15 % (w/v) magnesium sulphate
1 % (w/v) agar
1 % (v/v) glycerol
pH 7.0
Sterilise at 121 °C/15 min.
Ribose Ornithine Deoxycholate Medium for the Isolation
of Yersinia ruckeri (Rodgers 1992 )
0.3 % (w/v) yeast extract
0.1 % (w/v) sodium deoxycholate
0.5 % (w/v) sodium chloride
0.68 % (w/v) sodium thiosulphate
0.08 % (w/v) ferric ammonium citrate
0.75 % (w/v) maltose
0.375 % (w/v) ribose
0.375 % (w/v) ornithine hydrochloride
0.1 % (w/v) sodium dodecyl sulphate
0.008 % (w/v) phenol red
1.25 % (w/v) agar
pH 7.4; 10 ml of a fi ltered (0.22 μm) solution containing 0.5 g sucrose/ml should be
added after the basal medium has been autoclaved (121 °C/15 min) and cooled
to 50 °C.
Rimler-Shotts Medium (Shotts and Rimler 1973 )
0.05 % (w/v) L-lysine hydrochloride
0.65 % (w/v) L-ornithine hydrochloride
0.35 % (w/v) maltose
0.68 % (w/v) sodium thiosulphate
0.03 % (w/v) L-cysteine hydrochloride
0.003 % (w/v) bromothymol blue
0.08 % (w/v) ferric ammonium citrate
0.1 % (w/v) sodium deoxycholate
0.0005 % (w/v) novobiocin
12 Isolation/Detection