604
Isolation
Individual diseased eggs were placed on marine agar with incubation at 28 °C for
2 days (Nelson and Ghiorse 1999 ).
Characteristics of the Pathogen
An isolate, coined Cura-d, from Amblyglyphidodon curacao eggs was identifi ed by
16S rDNA sequencing as Pseudoalteromonas piscicida.
Pathogenicity
Damsel fi sh eggs challenged with a culture resulted in enhanced mortalities, compared to those of uninfected controls (Nelson and Ghiorse 1999 ).
Pseudoalteromonas undina
Characteristics of the Disease
The organism has been recovered from sea bass and sea bream with or without any
clinical signs of disease in Spain (Pujalte et al. 2007 ).
Isolation
Cultures were achieved using inocula from head kidney or liver by use of marine
agar with incubation at 20–25 °C for up to 10 days (Pujalte et al. 2007 ).
Characteristics of the Pathogen
One strain, U58, isolated from diseased sea bass was considered to be pathogenic to
fi sh albeit in high doses, and equated with Pseudoalteromonas undina because of
the 16S rDNA sequence homology (Pujalte et al . 2007 ).
Box 11.1: Pseudoalteromonas piscicida
Colonies on marine 2216E agar are 3–6 mm in diameter and orange to dark
orange in colour (the centres are often white) with the pigment diffusing into
the agar. Cells comprise oxidative Gram-negative polarly fl agellated rods,
which utilise fructose, maltose, mannose and sucrose but not L-threonine, and
contain intracellular granules but not poly ß-hydroxybutyrate. Growth occurs
at 40 °C (Nelson and Ghiorse 1999 ).
11 Miscellaneous Pathogens
Isolation
Individual diseased eggs were placed on marine agar with incubation at 28 °C for
2 days (Nelson and Ghiorse 1999 ).
Characteristics of the Pathogen
An isolate, coined Cura-d, from Amblyglyphidodon curacao eggs was identifi ed by
16S rDNA sequencing as Pseudoalteromonas piscicida.
Pathogenicity
Damsel fi sh eggs challenged with a culture resulted in enhanced mortalities, compared to those of uninfected controls (Nelson and Ghiorse 1999 ).
Pseudoalteromonas undina
Characteristics of the Disease
The organism has been recovered from sea bass and sea bream with or without any
clinical signs of disease in Spain (Pujalte et al. 2007 ).
Isolation
Cultures were achieved using inocula from head kidney or liver by use of marine
agar with incubation at 20–25 °C for up to 10 days (Pujalte et al. 2007 ).
Characteristics of the Pathogen
One strain, U58, isolated from diseased sea bass was considered to be pathogenic to
fi sh albeit in high doses, and equated with Pseudoalteromonas undina because of
the 16S rDNA sequence homology (Pujalte et al . 2007 ).
Box 11.1: Pseudoalteromonas piscicida
Colonies on marine 2216E agar are 3–6 mm in diameter and orange to dark
orange in colour (the centres are often white) with the pigment diffusing into
the agar. Cells comprise oxidative Gram-negative polarly fl agellated rods,
which utilise fructose, maltose, mannose and sucrose but not L-threonine, and
contain intracellular granules but not poly ß-hydroxybutyrate. Growth occurs
at 40 °C (Nelson and Ghiorse 1999 ).
11 Miscellaneous Pathogens