566
oxolinic acid was partially successful. However, the presence of virus undoubtedly
complicated the chemotherapeutic regime (Lupiani et al. 1989 ). In a subsequent
study, Angulo et al. ( 1994 ) reported success with fl umequine as a feed additive.
Vibrio tapetis
Characteristics of the Disease
Although V. tapetis is primarily the cause of brown ring disease in bivalves, the
organism was recovered from the kidney of dead and moribund corkwing wrasse in
Norway (Jensen et al. 2003 ) and wild-caught Dover sole with vesicular skin lesions
(Declercq et al. 2015 ). The disease signs centred on inappetance, reduced swimming activity, and in some cases surface ulceration on corkwing wrasse (Jensen
et al. 2003 ), circular grey-white skin discolouration leading to ulceration in Dover
sole (Declercq et al. 2015 ).
Isolation
Jensen et al. ( 2003 ) used nutrient agar supplemented with 5 % (v/v) sheep blood and
1.5 % (w/v) NaCl, which was inoculated with kidney tissue and incubated aerobically at 15 °C for up to 7 days.
Characteristics of the Pathogen
A culture was obtained from diseased corkwing wrasse in Norway. The characteristics were, as follows:
There was 78.4 % DNA: DNA re-association with a named reference culture of
V. tapetis (Jensen et al. 2003 ).
Box 10.18: Vibrio tapetis
The culture comprises motile, Gram-negative fermentative rods that produce
oxidase but not arginine dihydrolase, and require NaCl. The Voges Proskauer
reaction is negative. Growth does not occur at 37 °C. Blood is not degraded.
Glucose, ribose and sucrose are utilised, but not mannitol or sorbitol. The
G + C ratio of the DNA is 43.8 mols% (Jensen et al. 2003 ).
10 Vibrios
oxolinic acid was partially successful. However, the presence of virus undoubtedly
complicated the chemotherapeutic regime (Lupiani et al. 1989 ). In a subsequent
study, Angulo et al. ( 1994 ) reported success with fl umequine as a feed additive.
Vibrio tapetis
Characteristics of the Disease
Although V. tapetis is primarily the cause of brown ring disease in bivalves, the
organism was recovered from the kidney of dead and moribund corkwing wrasse in
Norway (Jensen et al. 2003 ) and wild-caught Dover sole with vesicular skin lesions
(Declercq et al. 2015 ). The disease signs centred on inappetance, reduced swimming activity, and in some cases surface ulceration on corkwing wrasse (Jensen
et al. 2003 ), circular grey-white skin discolouration leading to ulceration in Dover
sole (Declercq et al. 2015 ).
Isolation
Jensen et al. ( 2003 ) used nutrient agar supplemented with 5 % (v/v) sheep blood and
1.5 % (w/v) NaCl, which was inoculated with kidney tissue and incubated aerobically at 15 °C for up to 7 days.
Characteristics of the Pathogen
A culture was obtained from diseased corkwing wrasse in Norway. The characteristics were, as follows:
There was 78.4 % DNA: DNA re-association with a named reference culture of
V. tapetis (Jensen et al. 2003 ).
Box 10.18: Vibrio tapetis
The culture comprises motile, Gram-negative fermentative rods that produce
oxidase but not arginine dihydrolase, and require NaCl. The Voges Proskauer
reaction is negative. Growth does not occur at 37 °C. Blood is not degraded.
Glucose, ribose and sucrose are utilised, but not mannitol or sorbitol. The
G + C ratio of the DNA is 43.8 mols% (Jensen et al. 2003 ).
10 Vibrios