562
Isolation
Marine agar and TCBS were used with incubation at 25 °C for 48 h (Xie et al.
2007 ).
Characteristics of the Pathogen
A total of 6 cultures were recovered from livers of diseased Japanese sea bass:
Generally, the phenotypic characteristics were in common with the description
of Macián et al. ( 2004 ). Sequencing of the 16S rDNA gene revealed the highest
homology (99.3 %) to V. ponticus (Xie et al. 2007 ).
Pathogenicity
Cultures were pathogenic to Japanese sea bass following challenge by i.p. and i.m.
injection with the LD 50 doses reported as 2.5–3.2 × 10
3 CFU/fi sh (Xie et al. 2007 ).
Dead and moribund fi sh displayed ulceration and subcutaneous bleeding (Xie et al.
2007 ) .
Vibrio scophthlalmi
In 2005, A massive mortality occurred in farmed olive fl ounder ( Paralichthys olivaceus ) in Korea during 2005, from which fi ve isolates were recovered from diseased
fi sh (Qiao et al. 2012 ).
Box 10.15: Vibrio ponticus
Cultures on marine agar are white, and comprise Gram-negative motile,
curved rods that produce oxidase, arginine dihydrolase and indole but not
lysine or ornithine decarboxylase. The Voges Proskauer reaction is negative.
Growth occurs at 25 and 37 °C but not at 4 or 40 °C, and in 2–8 % but not 0 %
or 10 % (w/v) sodium chloride. Acid is produced from amygdalin, arabinose,
glucose and sucrose. 3-hydroxybutyrate is utilised. Nitrates are reduced to
nitrite (Xie et al. 2007 ).
10 Vibrios
Isolation
Marine agar and TCBS were used with incubation at 25 °C for 48 h (Xie et al.
2007 ).
Characteristics of the Pathogen
A total of 6 cultures were recovered from livers of diseased Japanese sea bass:
Generally, the phenotypic characteristics were in common with the description
of Macián et al. ( 2004 ). Sequencing of the 16S rDNA gene revealed the highest
homology (99.3 %) to V. ponticus (Xie et al. 2007 ).
Pathogenicity
Cultures were pathogenic to Japanese sea bass following challenge by i.p. and i.m.
injection with the LD 50 doses reported as 2.5–3.2 × 10
3 CFU/fi sh (Xie et al. 2007 ).
Dead and moribund fi sh displayed ulceration and subcutaneous bleeding (Xie et al.
2007 ) .
Vibrio scophthlalmi
In 2005, A massive mortality occurred in farmed olive fl ounder ( Paralichthys olivaceus ) in Korea during 2005, from which fi ve isolates were recovered from diseased
fi sh (Qiao et al. 2012 ).
Box 10.15: Vibrio ponticus
Cultures on marine agar are white, and comprise Gram-negative motile,
curved rods that produce oxidase, arginine dihydrolase and indole but not
lysine or ornithine decarboxylase. The Voges Proskauer reaction is negative.
Growth occurs at 25 and 37 °C but not at 4 or 40 °C, and in 2–8 % but not 0 %
or 10 % (w/v) sodium chloride. Acid is produced from amygdalin, arabinose,
glucose and sucrose. 3-hydroxybutyrate is utilised. Nitrates are reduced to
nitrite (Xie et al. 2007 ).
10 Vibrios