447
On the basis of DNA:DNA hybridisation, there is 26–42 % homology with T.
maritimum. Certainly, the strains are distinct from T. maritimum, but it would be
relevant
to
enquire
about
possible
relationships
with
other
cytophagas-fl exibacteria.
Tenacibaculum soleae
Characteristics of the Disease
The pathogen has been recovered from Senegalese sole ( Solea senegalensis ), wedge
sole ( Dicologoglossa cuneata ), brill ( Scophthalmus rhombus ) and turbot ( Psetta
maxima ) (López et al. 2010 ) and sea bass (Castro et al. 2014 ) in Spain. In brill and
wedge sole, heavy mortalities of up to 90 % occurred in juveniles; with 7–9 % deaths
in adults with disease signs centring on ulcers on the body and tail. In addition,
affected fi sh displayed haemorrhagic or pale livers, petechial on the fi nage, jaw, and
ventral body surface, anaemia, and erratic swimming (López et al. 2010 ).
Characteristics of the Pathogen
A single culture from sole ( Solea senegalensis ) in Spain was used to describe a new
species:
From 16S rRNA sequencing, the closest neighbours were T. ovolyticum and T.
aestuarii with 96.7 % homology to T. soleae (Piñeiro-Vidal et al. 2008b ).
Box 7.21: Tenacibaculum soleae
Yellow-pigmented [not fl exirubin pigment], strictly aerobic Gram-negative
rods of 2–25 × 0.5 μm in size, which demonstrate gliding movement. Optimal
growth occurs at 22–25 °C. Growth occurs in media containing 50–100 %
seawater, but not in media supplemented with sodium chloride. Catalase and
oxidase are produced, but not H 2 S or indole. Casein and gelatin are degraded,
but not starch or Tween 80. Neither L-proline, L-glutamate, sucrose, D-ribose,
D-galactose, D-glucose no L-tyrosine is utilised. Alkaline phosphatase, esterase lipase, cystine, leucine and valine arylamidase, lipase is produced on the
API ZYM system, but not α-chymotrypsin or trypsin. The G + C ratio of the
DNA was reported as 29.8 moles % (Piñeiro-Vidal et al. 2008b ).
Tenacibaculum soleae
On the basis of DNA:DNA hybridisation, there is 26–42 % homology with T.
maritimum. Certainly, the strains are distinct from T. maritimum, but it would be
relevant
to
enquire
about
possible
relationships
with
other
cytophagas-fl exibacteria.
Tenacibaculum soleae
Characteristics of the Disease
The pathogen has been recovered from Senegalese sole ( Solea senegalensis ), wedge
sole ( Dicologoglossa cuneata ), brill ( Scophthalmus rhombus ) and turbot ( Psetta
maxima ) (López et al. 2010 ) and sea bass (Castro et al. 2014 ) in Spain. In brill and
wedge sole, heavy mortalities of up to 90 % occurred in juveniles; with 7–9 % deaths
in adults with disease signs centring on ulcers on the body and tail. In addition,
affected fi sh displayed haemorrhagic or pale livers, petechial on the fi nage, jaw, and
ventral body surface, anaemia, and erratic swimming (López et al. 2010 ).
Characteristics of the Pathogen
A single culture from sole ( Solea senegalensis ) in Spain was used to describe a new
species:
From 16S rRNA sequencing, the closest neighbours were T. ovolyticum and T.
aestuarii with 96.7 % homology to T. soleae (Piñeiro-Vidal et al. 2008b ).
Box 7.21: Tenacibaculum soleae
Yellow-pigmented [not fl exirubin pigment], strictly aerobic Gram-negative
rods of 2–25 × 0.5 μm in size, which demonstrate gliding movement. Optimal
growth occurs at 22–25 °C. Growth occurs in media containing 50–100 %
seawater, but not in media supplemented with sodium chloride. Catalase and
oxidase are produced, but not H 2 S or indole. Casein and gelatin are degraded,
but not starch or Tween 80. Neither L-proline, L-glutamate, sucrose, D-ribose,
D-galactose, D-glucose no L-tyrosine is utilised. Alkaline phosphatase, esterase lipase, cystine, leucine and valine arylamidase, lipase is produced on the
API ZYM system, but not α-chymotrypsin or trypsin. The G + C ratio of the
DNA was reported as 29.8 moles % (Piñeiro-Vidal et al. 2008b ).
Tenacibaculum soleae