401
Isolation Generally, the aetiological agents may be readily recovered from diseased tissues on low-nutrient media, with incubation at 10–25 °C for 4–14 days.
Specialised media have been devised, of which cytophaga agar (Appendix in
Chap. 12 ; after Anacker and Ordal 1959 ) has received greatest use. This is suitable
for the isolation of Fla. hydatis, Fla. johnsoniae (specifi cally with incubation at
27 °C for 7 days; Carson et al. 1993 ), Fla. psychrophilum, Fla. branchiophilum and
Fla. columnare. The problem of overgrowth/out-competition by saprophytes has
been raised in connection with the recovery of the causal agent of fl avobacteriosis
with the advise that serial dilutions of the (external) samples are needed before plating techniques are used in order to maximise the possibility of recovering the actual
disease causing agent (Tiirola et al. 2002 ).
Chryseobacterium spp.
Chryseobacteria have been described that infected fi sh from the Great Lakes of
North America, and which were regarded as novel (Loch and Faisal 2015 ).
The isolation of 17 cultures was achieved from kidney, gills or fi ns using HsuShotts medium (Bullock et al. 1986 ) supplemented with 4 mg of neomycin/ml with
incubation at 22 °C for up to 7-days (Loch and Faisal 2015 ). Two of these isolates
were regarded as comprising novel taxa (Loch and Faisal 2015 ).
Chryseobacterium aahli
Characteristics of the Disease
Cultures were obtained during disease surveillance in 2009. The disease signs were
as expected of chryseobacteria, and included the presence of necrotic fi nage (Loch
and Faisal 2014b ).
Isolation
Diseased tissue, i.e. kidney and necrotic fi n, was inoculated onto Hsu–Shotts
medium (Bullock et al. 1986 ) with incubation at 22 °C for 72 h (Loch and Faisal
2014b ).
Chryseobacterium aahli
Isolation Generally, the aetiological agents may be readily recovered from diseased tissues on low-nutrient media, with incubation at 10–25 °C for 4–14 days.
Specialised media have been devised, of which cytophaga agar (Appendix in
Chap. 12 ; after Anacker and Ordal 1959 ) has received greatest use. This is suitable
for the isolation of Fla. hydatis, Fla. johnsoniae (specifi cally with incubation at
27 °C for 7 days; Carson et al. 1993 ), Fla. psychrophilum, Fla. branchiophilum and
Fla. columnare. The problem of overgrowth/out-competition by saprophytes has
been raised in connection with the recovery of the causal agent of fl avobacteriosis
with the advise that serial dilutions of the (external) samples are needed before plating techniques are used in order to maximise the possibility of recovering the actual
disease causing agent (Tiirola et al. 2002 ).
Chryseobacterium spp.
Chryseobacteria have been described that infected fi sh from the Great Lakes of
North America, and which were regarded as novel (Loch and Faisal 2015 ).
The isolation of 17 cultures was achieved from kidney, gills or fi ns using HsuShotts medium (Bullock et al. 1986 ) supplemented with 4 mg of neomycin/ml with
incubation at 22 °C for up to 7-days (Loch and Faisal 2015 ). Two of these isolates
were regarded as comprising novel taxa (Loch and Faisal 2015 ).
Chryseobacterium aahli
Characteristics of the Disease
Cultures were obtained during disease surveillance in 2009. The disease signs were
as expected of chryseobacteria, and included the presence of necrotic fi nage (Loch
and Faisal 2014b ).
Isolation
Diseased tissue, i.e. kidney and necrotic fi n, was inoculated onto Hsu–Shotts
medium (Bullock et al. 1986 ) with incubation at 22 °C for 72 h (Loch and Faisal
2014b ).
Chryseobacterium aahli