365
Presumably, effective chemotherapy could be achieved with one or more of these
compounds.
Yersinia intermedia
Characteristics of the Disease
Affected Atlantic salmon were of 40–50 g in weight, and were held at a water temperature of 5 °C. Disease signs included lazy movement with the fi sh congregating
at the surface of the water, darkening of the body pigment, tail erosion, haemorrhaging on the fl ank and abdominal infl ammation (Carson and Schmidtke 1993 ).
Isolation
Blood samples were cultured on Oxoid blood agar base supplemented with 7 %
(v/v) defi brinated sheep’s blood at 18 °C for 7 days (Carson and Schmidtke 1993 ).
After 48 h, the kidneys of two fi sh revealed the presence of dense growth, equated
with Yersinia intermedia. In addition, other bacteria were recovered including CLB
and Ps. fl uorescens.
Characteristics of the Pathogen
Characteristics matched the species description (Farmer and Kelley 1991 ) at 36 °C,
except that the fi sh-isolate utilised sodium citrate and was weakly positive in the
Voges Proskauer reaction (Carson and Schmidtke 1993 ).
Box 6.17: Yersinia intermedia
The culture comprises fermentative, motile (at 25 °C but not 36 °C) cells that
produce ß-galactosidase and indole, but not arginine dihydrolase, H 2 S, lysine
or ornithine decarboxylase or oxidase. Aesculin and urea are degraded. The
methyl red test and Voges Proskauer reaction (at 36 °C but not 25 °C) are positive. Nitrates are reduced. Acid is produced from glycerol, inositol, mannitol,
melibiose (at 36 °C but not 25 °C), rhamnose, sorbitol, sucrose, trehalose and
xylose, but not adonitol or lactose. Sodium citrate is utilised at 36 °C but not
25 °C (Carson and Schmidtke 1993 ).
Yersinia intermedia
Presumably, effective chemotherapy could be achieved with one or more of these
compounds.
Yersinia intermedia
Characteristics of the Disease
Affected Atlantic salmon were of 40–50 g in weight, and were held at a water temperature of 5 °C. Disease signs included lazy movement with the fi sh congregating
at the surface of the water, darkening of the body pigment, tail erosion, haemorrhaging on the fl ank and abdominal infl ammation (Carson and Schmidtke 1993 ).
Isolation
Blood samples were cultured on Oxoid blood agar base supplemented with 7 %
(v/v) defi brinated sheep’s blood at 18 °C for 7 days (Carson and Schmidtke 1993 ).
After 48 h, the kidneys of two fi sh revealed the presence of dense growth, equated
with Yersinia intermedia. In addition, other bacteria were recovered including CLB
and Ps. fl uorescens.
Characteristics of the Pathogen
Characteristics matched the species description (Farmer and Kelley 1991 ) at 36 °C,
except that the fi sh-isolate utilised sodium citrate and was weakly positive in the
Voges Proskauer reaction (Carson and Schmidtke 1993 ).
Box 6.17: Yersinia intermedia
The culture comprises fermentative, motile (at 25 °C but not 36 °C) cells that
produce ß-galactosidase and indole, but not arginine dihydrolase, H 2 S, lysine
or ornithine decarboxylase or oxidase. Aesculin and urea are degraded. The
methyl red test and Voges Proskauer reaction (at 36 °C but not 25 °C) are positive. Nitrates are reduced. Acid is produced from glycerol, inositol, mannitol,
melibiose (at 36 °C but not 25 °C), rhamnose, sorbitol, sucrose, trehalose and
xylose, but not adonitol or lactose. Sodium citrate is utilised at 36 °C but not
25 °C (Carson and Schmidtke 1993 ).
Yersinia intermedia