357
Providencia vermicola
Characteristics of the Disease
The organism was recovered from diseased farmed Indian major carp ( Labeo rohita )
with ulceration and reddish colouration on the abdominal surface, base of the pectoral fi ns and around the head. The disease occurred during August 2012 to July
2013, and resulted in heavy mortalities (Ramkumar et al. 2014 ).
Isolation
Liver, gill and ulcerative skin tissue was inoculated onto Aeromonas agar, nutrient
agar and TSA with incubation at room temperature for 24–48 h. Dominant growth
on TSA from ulcerative skin was used in the subsequent investigation (Ramkumar
et al. 2014 ).
Characteristics of the Pathogen
Epizootiology
The source of the pathogen was considered to be from handling, and from nearby
poultry and human faeces. This could mean the the organism was an opportunist
pathogen (Ramkumar et al. 2014 ).
Pathogenicity
Cultures were pathogenic in infectivity experiments using Indian major carp, and
challenge by immersion and i.m. injection (Ramkumar et al. 2014 )
Box 6.12: Providencia vermicola
Cultures exhibit brown soluble diffusible pigment, and contain Gram-negative
motile rods. Catalase but not arginine dihydrolase is produced. Nitrates are
reduced. Urea is not attacked. Acid is produced from L-arabinose, glucose,
inositol an D-mannose, but not from amygdalin, rhamnose or sucrose. There
is 99 % 16S rRNA homology with Providencia vermicola (Ramkumar et al.
2014 ).
Providencia vermicola
Providencia vermicola
Characteristics of the Disease
The organism was recovered from diseased farmed Indian major carp ( Labeo rohita )
with ulceration and reddish colouration on the abdominal surface, base of the pectoral fi ns and around the head. The disease occurred during August 2012 to July
2013, and resulted in heavy mortalities (Ramkumar et al. 2014 ).
Isolation
Liver, gill and ulcerative skin tissue was inoculated onto Aeromonas agar, nutrient
agar and TSA with incubation at room temperature for 24–48 h. Dominant growth
on TSA from ulcerative skin was used in the subsequent investigation (Ramkumar
et al. 2014 ).
Characteristics of the Pathogen
Epizootiology
The source of the pathogen was considered to be from handling, and from nearby
poultry and human faeces. This could mean the the organism was an opportunist
pathogen (Ramkumar et al. 2014 ).
Pathogenicity
Cultures were pathogenic in infectivity experiments using Indian major carp, and
challenge by immersion and i.m. injection (Ramkumar et al. 2014 )
Box 6.12: Providencia vermicola
Cultures exhibit brown soluble diffusible pigment, and contain Gram-negative
motile rods. Catalase but not arginine dihydrolase is produced. Nitrates are
reduced. Urea is not attacked. Acid is produced from L-arabinose, glucose,
inositol an D-mannose, but not from amygdalin, rhamnose or sucrose. There
is 99 % 16S rRNA homology with Providencia vermicola (Ramkumar et al.
2014 ).
Providencia vermicola