348
Characteristics of the Pathogen
By 16S rRNA sequencing there was 100 % homology with Ent. cloacae (Sekar
et al. 2008 ).
Pathogenicity
Bath challenge resulted with 8.6 × 10
7 cells/ml for 1 h when 100 % of the mullet died
within 5 days at 30 °C. An unspecifi ed cationic factor was linked to pathogenicity
(Sekar et al. 2008 ).
Escherichia vulneris
Characteristics of the Disease
An organism, subsequently identifi ed as Esch. vulneris, was fi rst isolated in 1994
from naturally infected balloon moly ( Poecilia sp.), silver moly ( Poecilia sp.) and
Caucasian carp ( Carassius sp.) from Turkey (Aydin et al. 1997 ). Clinical signs
included haemorrhagic lesions on the skin, pale gills, digestive tract full of bloody
exudate, haemorrhaging in the gonads, and yellow liver with hyperaemic areas
(Aydin et al. 1997 ).
Isolation
Isolates were recovered on TSA with incubation at 25 °C for 48 h (Aydin et al.
1997 ).
Box 6.5: Enterobacter cloacae
Cultures comprise Gram-negative rod-shaped fermentative organisms motile
rods. Catalase, arginine dihydrolase and ornithine decarboxylase is produced
but not H 2 S, lysine decarboxylase or oxidase (Sekar et al. 2008 ).
6 Enterobacteriaceae Representatives
Characteristics of the Pathogen
By 16S rRNA sequencing there was 100 % homology with Ent. cloacae (Sekar
et al. 2008 ).
Pathogenicity
Bath challenge resulted with 8.6 × 10
7 cells/ml for 1 h when 100 % of the mullet died
within 5 days at 30 °C. An unspecifi ed cationic factor was linked to pathogenicity
(Sekar et al. 2008 ).
Escherichia vulneris
Characteristics of the Disease
An organism, subsequently identifi ed as Esch. vulneris, was fi rst isolated in 1994
from naturally infected balloon moly ( Poecilia sp.), silver moly ( Poecilia sp.) and
Caucasian carp ( Carassius sp.) from Turkey (Aydin et al. 1997 ). Clinical signs
included haemorrhagic lesions on the skin, pale gills, digestive tract full of bloody
exudate, haemorrhaging in the gonads, and yellow liver with hyperaemic areas
(Aydin et al. 1997 ).
Isolation
Isolates were recovered on TSA with incubation at 25 °C for 48 h (Aydin et al.
1997 ).
Box 6.5: Enterobacter cloacae
Cultures comprise Gram-negative rod-shaped fermentative organisms motile
rods. Catalase, arginine dihydrolase and ornithine decarboxylase is produced
but not H 2 S, lysine decarboxylase or oxidase (Sekar et al. 2008 ).
6 Enterobacteriaceae Representatives