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Edwardsiella piscicida
Characteristics of the Disease
Edw. piscicida has been recognized in Asia, Europe and in catfi sh culture in the
southeastern USA (Griffi n et al. 2014 ). The organism was recovered from septicaemic farmed whitefi sh ( Coregonus lavaretus ) in Finland during 2000, 2002 and 2013
(Shafi ei et al. 2016 ). The fi sh displayed exophthalmia, haemorrhaging on the skin,
at the base of the fi ns, the gills, and in the muscle, swelling and hyperaemia of the
anal area, and internal haemorrhages, and enlarged kidney and spleen (Shafi ei et al.
2016 ).
Isolation
Isolation was achieved using TSA and 5 % (v/v) blood agar with incubation at 15 or
20 °C for 10 days (Shafi ei et al. 2016 ).
Characteristics of the Pathogen
Multilocus variable number tandem repeat analysis of 37 isolates resulted in the
delineation of fi ve major clusters consistent with their geographical origins
(Abayneh et al. 2014 ).
Box 6.4: Edwardsiella piscicida
The organism produces pinprick slightly convex, glistening colonies, that are
slightly ß-haemolytic on TSA + blood agar. Optimum growth is at 85–30 °C,
and not at 12 or 42 °C, and in 1–5 % (w/v) but not 6 % (w/v) sodium chloride.
Cultures contain Gram-negative, facultatively anaerobic, straight motile rods.
Catalase, H 2 S, indole, and lysine and ornithine dcarboxylases are produced,
but not arginine hydrolase, ß-galactosidase, oxidase or tryptophan deaminase.
The methyl red test is positive, but the Voges Proskauer reaction is negative
Acid is produced from glucose, but not from amygdalin, arabinose, inositol,
mannitol, melibiose, rhamnose, saccharose or sorbitol. Citrate is not utilized.
Neither gelatin nor urea is degraded (Abayneh et al. 2012 ).
Edwardsiella piscicida
Edwardsiella piscicida
Characteristics of the Disease
Edw. piscicida has been recognized in Asia, Europe and in catfi sh culture in the
southeastern USA (Griffi n et al. 2014 ). The organism was recovered from septicaemic farmed whitefi sh ( Coregonus lavaretus ) in Finland during 2000, 2002 and 2013
(Shafi ei et al. 2016 ). The fi sh displayed exophthalmia, haemorrhaging on the skin,
at the base of the fi ns, the gills, and in the muscle, swelling and hyperaemia of the
anal area, and internal haemorrhages, and enlarged kidney and spleen (Shafi ei et al.
2016 ).
Isolation
Isolation was achieved using TSA and 5 % (v/v) blood agar with incubation at 15 or
20 °C for 10 days (Shafi ei et al. 2016 ).
Characteristics of the Pathogen
Multilocus variable number tandem repeat analysis of 37 isolates resulted in the
delineation of fi ve major clusters consistent with their geographical origins
(Abayneh et al. 2014 ).
Box 6.4: Edwardsiella piscicida
The organism produces pinprick slightly convex, glistening colonies, that are
slightly ß-haemolytic on TSA + blood agar. Optimum growth is at 85–30 °C,
and not at 12 or 42 °C, and in 1–5 % (w/v) but not 6 % (w/v) sodium chloride.
Cultures contain Gram-negative, facultatively anaerobic, straight motile rods.
Catalase, H 2 S, indole, and lysine and ornithine dcarboxylases are produced,
but not arginine hydrolase, ß-galactosidase, oxidase or tryptophan deaminase.
The methyl red test is positive, but the Voges Proskauer reaction is negative
Acid is produced from glucose, but not from amygdalin, arabinose, inositol,
mannitol, melibiose, rhamnose, saccharose or sorbitol. Citrate is not utilized.
Neither gelatin nor urea is degraded (Abayneh et al. 2012 ).
Edwardsiella piscicida