202
transcriptomics or proteomics will further deepen our understanding on system
biology and to discover new biomolecules and metabolic pathways that are crucial
for survival process of marine macrophytes in response to global climate change.
Acknowledgement LC-MS analysis and tentative identification of molecules using MA database
was carried out by UK and Dr. Adrian Lutz, Metabolomics Australia, School of BioSciences,
University of Melbourne. Tentative annotations using Metlin database and interpretation of mass
spectra was carried out by UK. MK collected the seagrass samples and processed them for GC and
LC-MS analysis. GC-MS profiling was carried out by Dorothee R Hahne, UWA Centre for
Metabolomics, The University of Western Australia. MK is grateful to the Australian Research
Council for awarding him Discovery Early Career Research Award (DECRA Fellowship,
DE150100461-2015).
References
Achamlale S, Rezzonico B, Grignon-Dubois M (2009) Rosmarinic acid from beach waste: isolation and HPLC quantification in Zostera detritus from Arcachon lagoon. Food Chem
113(4):878–883
Barros L, Dueñas M, Dias MI, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2013) Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions.
Food Chem 136(1):1–8
Cuny P, Serve L, Jupin H, Boudouresque C-F (1995) Water soluble phenolic compounds of the
marine phanerogam Posidonia oceanica in a Mediterranean area colonised by the introduced
chlorophyte Caulerpa taxifolia. Aquat Bot 52(3):237–242
Custódio L, Laukaityte S, Engelen AH, Rodrigues MJ, Pereira H, Vizetto-Duarte C et al (2016)
A comparative evaluation of biological activities and bioactive compounds of the seagrasses
Zostera marina and Zostera noltei from southern Portugal. Nat Prod Res 30(6):724–728
De Leeuw JW, Irene W, Rijpstra C, Nienhuis PH (1995) Free and bound fatty acids and hydroxy fatty
acids in the living and decomposing eelgrass Zostera marina L. Org Geochem 23(8):721–728
Duarte CM, Chiscano CL (1999) Seagrass biomass and production: a reassessment. Aquat Bot
65(1–4):159–174
Enerstvedt KH, Jordheim M, Andersen M (2016) Isolation and identification of flavonoids found
in Zostera marina collected in Norwegian coastal waters. Am J Plant Sci 7(7):1163–1172
Engle JM, Miller KA (2003) Distribution and morphology of eelgrass (Zoster marina L.) at the
California Channel Islands. In: Garcelon DK, Schwemm CA (eds) Sixth California Island symposium. Institute for Wildlife Studies, Arcata, pp 1584–1590
Farag MA, Porzel A, Wessjohann LA (2012) Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC–MS, LC–MS and 1D NMR
techniques. Phytochemistry 76:60–72
Gillan FT, Hogg RW, Drew EA (1984) The sterol and fatty acid compositions of seven tropical
seagrasses from North Queensland, Australia. Phytochemistry 23(12):2817–2821
Grignon-Dubois M, Rezzonico B (2012) First phytochemical evidence of chemotypes for the seagrass Zostera noltii. Plants 1(1):27–38
Gu J, Weber K, Klemp E, Winters G, Franssen SU, Wienpahl I et al (2012) Identifying core features of adaptive metabolic mechanisms for chronic heat stress attenuation contributing to systems robustness. Integr Biol 4(5):480–493
Hasler-Sheetal H, Castorani MCN, Glud RN, Canfield DE, Holmer M (2016) Metabolomics
reveals cryptic interactive effects of species interactions and environmental stress on nitrogen
and sulfur metabolism in seagrass. Environ Sci Technol 50(21):11602–11609
U. Kuzhiumparambil et al.
transcriptomics or proteomics will further deepen our understanding on system
biology and to discover new biomolecules and metabolic pathways that are crucial
for survival process of marine macrophytes in response to global climate change.
Acknowledgement LC-MS analysis and tentative identification of molecules using MA database
was carried out by UK and Dr. Adrian Lutz, Metabolomics Australia, School of BioSciences,
University of Melbourne. Tentative annotations using Metlin database and interpretation of mass
spectra was carried out by UK. MK collected the seagrass samples and processed them for GC and
LC-MS analysis. GC-MS profiling was carried out by Dorothee R Hahne, UWA Centre for
Metabolomics, The University of Western Australia. MK is grateful to the Australian Research
Council for awarding him Discovery Early Career Research Award (DECRA Fellowship,
DE150100461-2015).
References
Achamlale S, Rezzonico B, Grignon-Dubois M (2009) Rosmarinic acid from beach waste: isolation and HPLC quantification in Zostera detritus from Arcachon lagoon. Food Chem
113(4):878–883
Barros L, Dueñas M, Dias MI, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2013) Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions.
Food Chem 136(1):1–8
Cuny P, Serve L, Jupin H, Boudouresque C-F (1995) Water soluble phenolic compounds of the
marine phanerogam Posidonia oceanica in a Mediterranean area colonised by the introduced
chlorophyte Caulerpa taxifolia. Aquat Bot 52(3):237–242
Custódio L, Laukaityte S, Engelen AH, Rodrigues MJ, Pereira H, Vizetto-Duarte C et al (2016)
A comparative evaluation of biological activities and bioactive compounds of the seagrasses
Zostera marina and Zostera noltei from southern Portugal. Nat Prod Res 30(6):724–728
De Leeuw JW, Irene W, Rijpstra C, Nienhuis PH (1995) Free and bound fatty acids and hydroxy fatty
acids in the living and decomposing eelgrass Zostera marina L. Org Geochem 23(8):721–728
Duarte CM, Chiscano CL (1999) Seagrass biomass and production: a reassessment. Aquat Bot
65(1–4):159–174
Enerstvedt KH, Jordheim M, Andersen M (2016) Isolation and identification of flavonoids found
in Zostera marina collected in Norwegian coastal waters. Am J Plant Sci 7(7):1163–1172
Engle JM, Miller KA (2003) Distribution and morphology of eelgrass (Zoster marina L.) at the
California Channel Islands. In: Garcelon DK, Schwemm CA (eds) Sixth California Island symposium. Institute for Wildlife Studies, Arcata, pp 1584–1590
Farag MA, Porzel A, Wessjohann LA (2012) Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC–MS, LC–MS and 1D NMR
techniques. Phytochemistry 76:60–72
Gillan FT, Hogg RW, Drew EA (1984) The sterol and fatty acid compositions of seven tropical
seagrasses from North Queensland, Australia. Phytochemistry 23(12):2817–2821
Grignon-Dubois M, Rezzonico B (2012) First phytochemical evidence of chemotypes for the seagrass Zostera noltii. Plants 1(1):27–38
Gu J, Weber K, Klemp E, Winters G, Franssen SU, Wienpahl I et al (2012) Identifying core features of adaptive metabolic mechanisms for chronic heat stress attenuation contributing to systems robustness. Integr Biol 4(5):480–493
Hasler-Sheetal H, Castorani MCN, Glud RN, Canfield DE, Holmer M (2016) Metabolomics
reveals cryptic interactive effects of species interactions and environmental stress on nitrogen
and sulfur metabolism in seagrass. Environ Sci Technol 50(21):11602–11609
U. Kuzhiumparambil et al.