213
In the laboratory tests, fl uorescent images of
some deep-sea species were obtained using a
UV-LED light with the wavelength of 375 nm
and a violet laser with the wavelength of 405 nm.
In the fi eld trial, the fl uorescent images of a biological community on hydrothermal vents were
obtained using a UV-LED light mounted on the
ROV, and three species were distinguished on
the fl uorescent image. These results implied that
the bio-fl uorescent characteristics could be
applied to in situ observation for deep-sea
organisms.
In the near future, Raman spectroscopy will be
applied to deep-sea benthos, hydrothermal
plumes and ore investigation as a new in situ
observation method in addition to UV-excited
fl uorescent image investigation.
Acknowledgements This work was supported by JSPS
KAKENHI Grant Number 12929746. The authors wish to
thank the Enoshima Aquarium for the fl uorescent observation tests. The authors are also grateful to Prof.
B. Thornton at University of Tokyo and the
‘ Hyper- Dolphin’ team at JAMSTEC for the deep-sea fl uorescence observations.
References
IPCC: annual report 4, Working Group 2. http://www.
ipcc.ch/publications_and_data/ar4/wg2/en/contents.
html (2007)
JAMSTEC Press Release. http://www.jamstec.go.jp/j/
about/press_release/20120323/ (2012). (in Japanese)
JAMSTEC Press Release: electricity generation and illumination via an environmental fuel cell in deep-sea
hydrothermal vents. http://www.jamstec.go.jp/j/about/
press_release/20130903/ (2013). (in Japanese)
JAMSTEC Web page: 3,000 m class remotely operated
vehicle Hyper-Dolphin. http://www.jamstec.go.jp/e/
about/equipment/ships/hyperdolphin.html (2013)
Takai K, Kawagucci S, Miyazaki J, Watsuji T, Ishibashi J,
Yamamoto H, Nozaki T, Kashiwabara T, Shibuya T
(2013) Post-drilling hydrothermal vent and associated
biological activities seen through artifi cial hydrothermal vents in the Iheya North fi eld. Okinawa Trough.
In: International symposium on underwater technology 2013
The fi nal report of the fi rst term in development program
of hydrothermal deposits. http://www.meti.go.jp/press
/2013/07/20130705003/20130705003-2.pdf (2013).
(in Japanese)
Weiss RF, Lonsdale P, Lupton JE, Bainbridge AE, Craig H
(1977) Hydrothermal plumes in the Galapagos Rift.
Nat Mag 267(5612):600–603
Use of Bio-fl uorescent Characteristics for Ecosystem Monitoring on Hydrothermal Deposits
In the laboratory tests, fl uorescent images of
some deep-sea species were obtained using a
UV-LED light with the wavelength of 375 nm
and a violet laser with the wavelength of 405 nm.
In the fi eld trial, the fl uorescent images of a biological community on hydrothermal vents were
obtained using a UV-LED light mounted on the
ROV, and three species were distinguished on
the fl uorescent image. These results implied that
the bio-fl uorescent characteristics could be
applied to in situ observation for deep-sea
organisms.
In the near future, Raman spectroscopy will be
applied to deep-sea benthos, hydrothermal
plumes and ore investigation as a new in situ
observation method in addition to UV-excited
fl uorescent image investigation.
Acknowledgements This work was supported by JSPS
KAKENHI Grant Number 12929746. The authors wish to
thank the Enoshima Aquarium for the fl uorescent observation tests. The authors are also grateful to Prof.
B. Thornton at University of Tokyo and the
‘ Hyper- Dolphin’ team at JAMSTEC for the deep-sea fl uorescence observations.
References
IPCC: annual report 4, Working Group 2. http://www.
ipcc.ch/publications_and_data/ar4/wg2/en/contents.
html (2007)
JAMSTEC Press Release. http://www.jamstec.go.jp/j/
about/press_release/20120323/ (2012). (in Japanese)
JAMSTEC Press Release: electricity generation and illumination via an environmental fuel cell in deep-sea
hydrothermal vents. http://www.jamstec.go.jp/j/about/
press_release/20130903/ (2013). (in Japanese)
JAMSTEC Web page: 3,000 m class remotely operated
vehicle Hyper-Dolphin. http://www.jamstec.go.jp/e/
about/equipment/ships/hyperdolphin.html (2013)
Takai K, Kawagucci S, Miyazaki J, Watsuji T, Ishibashi J,
Yamamoto H, Nozaki T, Kashiwabara T, Shibuya T
(2013) Post-drilling hydrothermal vent and associated
biological activities seen through artifi cial hydrothermal vents in the Iheya North fi eld. Okinawa Trough.
In: International symposium on underwater technology 2013
The fi nal report of the fi rst term in development program
of hydrothermal deposits. http://www.meti.go.jp/press
/2013/07/20130705003/20130705003-2.pdf (2013).
(in Japanese)
Weiss RF, Lonsdale P, Lupton JE, Bainbridge AE, Craig H
(1977) Hydrothermal plumes in the Galapagos Rift.
Nat Mag 267(5612):600–603
Use of Bio-fl uorescent Characteristics for Ecosystem Monitoring on Hydrothermal Deposits