ParaUel Genotyping of Different Genes: A Rapid Real-Time peR Approach B
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Fig.2A-G. Melting curve analysis of the amplified gene fragments shown in Fig. 1. Melting curve
analysis was performed by plotting the negative first derivative of the fluorescence (F) with respect
to temperature (1') against temperature [-(dF/dT) vs TJ. A Heterozygous HFE H63D mutant (curve 2,
control DNA), homozygous HFE wild type (curve 1, sample DNA). B Homozygous HFE C282Y
mutant (curve 1, control DNA), heterozygous HFE C282Y mutant (curve 2, control DNA), homozygous HFE wild type (curve 3, sample DNA). C Homozygous AAT PiZZ genotype (curve 1, control
DNA), heterozygous AAT PiMZ genotype (curve 2, control DNA), homozygous AAT (PiMM) wild
type (curve 3, sample DNA). D Heterozygous factor V mutant (curve 1, control DNA), homozygous
factor V wild type (curve 2, sample DNA). E Heterozygous APOBlOO G9775A mutant (curve 1, control DNA), heterozygous APOBlOO C9774T mutant (curve 2, control DNA), homozygous APOBlOO
wild type (curve 3, sample DNA). F Heterozygous APOE C3932T mutant (curve 1, control DNA),
homozygous APOE C3932T mutant (curve 2, sample DNA). G Heterozygous APOE C4070T mutant
(curve 1, control DNA), heterozygous APOE C4070T mutant (curve 2, sample DNA)
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