Parallel Genotyping of Different Genes: A Rapid Real-Time peR Approach . .
parallel real-time peR
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Fig. lA, B. Online fluorescence curves of parallel IightCyder-assisted PCR amplification of HFE,
AAT,jactor V, APOBlOO and APOE gene fragments from a representative patient sample. Using
LCRed640-labeled hybridization probes, fluorescence was measured in channel 2 (A). For the
detection of the APOE C4070T mutation, LCRed70S-labeled hybridization probes were used and
fluorescence was measured in channel 3 (B).A negative control was run with each mutation analysis. As indicated in Fig. 2, positive controls for each mutation analysis were amplified as follows:
heterogeneous control DNA in case of the HFE H63D,jactor V, APOBlOO and APOE mutations,
heterogeneous and homogeneous control DNA in case of the HFE C282Y and AAT mutations