Table of Contents
Introduction for Genetics and Oncology Volume
Rapid Cycle Real-Time PCR
Methods and Applications ..........................................
1
NATARAJAN SIVASUBRAMANIAN
Part I
Methods Useful in Genetics and Oncology
Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples
by Real-Time Quantitative PCR Technology Using the LightCycier Instrument
5
XI ZHU, GEORG BAUMGARTEN, FENG WANG, ZIAD DIBBS,
ABHINAV DIWAN, GUILLERMO TORRE-AMIONE, DOUGLAS L. MANN,
NATARAJAN SIVASUBRAMANIAN
Quantitative Two-Step RT -PCR for the Detection of Human ABCA 1 Transporter
on LightCycier Using Hybridization Probes and External Standards ...........
15
DANUTA KIELAR, WOLFGANG DIETMAIER, THOMAS LANG MANN,
CHARALAMPOS ASLANIDIS, MARIO PROBST, MAREK NARUSZEWICZ,
GERD SCHMITZ
Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene
27
NATHALIE PIERI-BALANDRAUD, JEAN ROUDIER, CHANTAL ROUDIER
Genotyping by Guanosine-Dependent Quenching
of Single-Labeled Fluorescein Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
35
ANDREW O. CROCKETT
Limitations of Melting Curve Analysis Using SYBR Green I -
Fragment Differentiation and Mutation Detection in the CFTR-Gene
S. KLEINLE, K. TABITI AND S. GALLATI
SYBR Green I Analysis of the Trinucleotide Repeat Responsible
47
for Huntington's Disease. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
57
CAMERON N. GUNDRY, CARL T. WITTWER
•