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Methods Useful in Genetics and Oncology
References
1. Bernard PS, Ajioka RS, Kushner JP, Wittwer CT (1998) Homogeneous multiplex genotyping
of hemochromatosis mutations with fluorescent hybridization probes. Am J Pathol
153:lO55-lO61
2. Gundry CN, Bernard PS, Herrmann MG, Reed GH, Wittwer CT (1999) Rapid F508dei and
F508C assay using fluorescent hybridization probes. Genet Test 3:365-370
3. Bernard PS, Lay MJ, Wittwer CT (1998) Integrated amplification and detection of the C677T
point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance
energy transfer and probe melting curves. Anal Biochem 255:lO1-lO7
4. Lay MJ, Wittwer CT (1997) Real-time fluorescence genotyping of factor V Leiden during rapid cycle PCR. Clin Chern 43:2262-2267
5. Von Ahsen N, Schutz E, Armstrong VW, Oellerich M (1999) Rapid detection of prothrombotic mutation of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler. Clin Chern
45:694-696
6. Lee LG, Connell CR, Bloch W (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucl Acids Res 21:3761-3766
7. Tyagi S, Kramer FR (1996) Molecular beacons - probes that fluoresce upon hybridization.
Nat BiotechnoI14:303-308
8. Whitcombe D, Theaker J, Guy SP, Brown T, Little S (1999) Detection of PCR products using
self-probing amplicons and fluorescence. Nat BiotechnoI17:804-807
9. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22:130-138
lO. Crockett AO, Wittwer CT (2001) Fluorescein-labeled oligonucleotides for real-time PCR:
using the inherent quenching of deoxyguanosine nucleotides. Anal Biochem 290:89-97
11. Thomas SM, Moreno RF, Tilzer LL (1989) DNA extraction with organic solvents in gel barrier tubes. Nucl Acids Res 17:5411
12. Borer PN (1975) In: Fasman, GD (ed) Handbook of Biochemistry and Molecular Biology, 3rd
edn. (Nucleic acids, vol 1 ), CRC Press, Boca Raton, pp 589
13. Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GJ, den Heijer M,
Kluijtmans LA, van den Heuvel LP, Rozen, R (1995) A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat Genet
lO:111-113
14. Bertina RM, Koeleman BPC, Koster T, Rosendaal FR, Dirven RJ, deRonde H, van der Velden
PA, Reitsma PH (1994) Mutation in blood coagulation factor V associated with resistance to
activated protein C. Nature 369:64-67
15. Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent
dyes at opposite ends provide a quenched probe system useful for detecting PCR product
and nucleic acid hybridization. PCR Meth AppI4:357-362
16. Singh KK, Rucker T, Hanne A, Parwaresch R, Krupp G (2000) Fluorescence polarization for
monitoring ribozyme reactions in real time. BioTechniques 29:344-351
Methods Useful in Genetics and Oncology
References
1. Bernard PS, Ajioka RS, Kushner JP, Wittwer CT (1998) Homogeneous multiplex genotyping
of hemochromatosis mutations with fluorescent hybridization probes. Am J Pathol
153:lO55-lO61
2. Gundry CN, Bernard PS, Herrmann MG, Reed GH, Wittwer CT (1999) Rapid F508dei and
F508C assay using fluorescent hybridization probes. Genet Test 3:365-370
3. Bernard PS, Lay MJ, Wittwer CT (1998) Integrated amplification and detection of the C677T
point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance
energy transfer and probe melting curves. Anal Biochem 255:lO1-lO7
4. Lay MJ, Wittwer CT (1997) Real-time fluorescence genotyping of factor V Leiden during rapid cycle PCR. Clin Chern 43:2262-2267
5. Von Ahsen N, Schutz E, Armstrong VW, Oellerich M (1999) Rapid detection of prothrombotic mutation of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler. Clin Chern
45:694-696
6. Lee LG, Connell CR, Bloch W (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucl Acids Res 21:3761-3766
7. Tyagi S, Kramer FR (1996) Molecular beacons - probes that fluoresce upon hybridization.
Nat BiotechnoI14:303-308
8. Whitcombe D, Theaker J, Guy SP, Brown T, Little S (1999) Detection of PCR products using
self-probing amplicons and fluorescence. Nat BiotechnoI17:804-807
9. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22:130-138
lO. Crockett AO, Wittwer CT (2001) Fluorescein-labeled oligonucleotides for real-time PCR:
using the inherent quenching of deoxyguanosine nucleotides. Anal Biochem 290:89-97
11. Thomas SM, Moreno RF, Tilzer LL (1989) DNA extraction with organic solvents in gel barrier tubes. Nucl Acids Res 17:5411
12. Borer PN (1975) In: Fasman, GD (ed) Handbook of Biochemistry and Molecular Biology, 3rd
edn. (Nucleic acids, vol 1 ), CRC Press, Boca Raton, pp 589
13. Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GJ, den Heijer M,
Kluijtmans LA, van den Heuvel LP, Rozen, R (1995) A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat Genet
lO:111-113
14. Bertina RM, Koeleman BPC, Koster T, Rosendaal FR, Dirven RJ, deRonde H, van der Velden
PA, Reitsma PH (1994) Mutation in blood coagulation factor V associated with resistance to
activated protein C. Nature 369:64-67
15. Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent
dyes at opposite ends provide a quenched probe system useful for detecting PCR product
and nucleic acid hybridization. PCR Meth AppI4:357-362
16. Singh KK, Rucker T, Hanne A, Parwaresch R, Krupp G (2000) Fluorescence polarization for
monitoring ribozyme reactions in real time. BioTechniques 29:344-351