Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene E'I
COMPARISON OF CONCENTRATIONS ON GEL AND WITH LlGHTCYCLER
600
500
'3
i .00
-' w
III
z
0
z 300
0
;:::
~
.... z w t..> 200
z
0
t..>
1I d
1
100
0
0
~
100
1~
200
CONCENTRATION WITH LlGHTCYClER (ng/41'1)
Fig. 5.Comparison of concentrations evaluated on gel and with lightcycler. Concentrations of
six samples evaluated with LightCycler (colored squares) are plotted against concentrations evaluated on gel (error bars)
References
1. Sambrook J, Fritsch EF, Maniatis T (1989) Appendix E: commonly used techniques in
molecular cloning. E5: quantitation of DNA and RNA. In: Molecular cloning. A laboratory
manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
2. Fitzgibbon J, Gillett GT, Woodwark KJ, Boyle JM, Wolfe J, Povey S (1993) Mapping of RXRB
to human chromosome 6p21.3. Ann Hum Genet 57:203-209
3. Jabs WJ, Hennig H, Fittel M, Pethig K, Smets F, Bucsky P, Kirchner H, Wagner HJ (2001) Normalized quantification by real-time PCR of Epstein Barr virus load in patients at risk for
transplant lymphoproliferative disorders. J Clin Microbiol 39:564-569
2~
300