Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene . , .
MELTING CURVE
3D,O
Z6,O
ZO,O
i
~
....
i
11,0
•
.. t::
. .. 10,0
! 0
"
ii:
5,0
0,.
71 .5
72."
'''.0
n .•
77,2
11,'
aO,.4
12,.
n ,'
.s,O
Temperatura (C·)
Fig. 1. SYBR Green I melting curve of the amplified retinoic X receptor B gene. The negative
derivative of fluorescence versus temperature is shown. All DNA dilutions show the same profIle with an average T m of 86.7
45
40
•
35
30
ffi
I 25
::I
R'.O,9901
Z
w
620
,..
U
IS
10
5
0
0,038
0,075
0.15
0,31
0,83
1,25
2,5
10
CONCENTRATION In9/4~1J
Fig. 2. Standard curve using SYBR Green I. A dilution series of human genomic DNA was used
as standard DNA template for amplification of a RXRB lSO-bp fragment. The calibration curve
shows the logarithmic plot of the RXRB template concentration versus cycle number
II,.
n ,o
20
40
80