~
'a
:::&.
...
~
l&V
• u c
I
J
"' "
~
~ ...
Yo
~
V
• u
8
• !
8
~
0.2tO~ -
020022 -
Analysis of Microsatellite Instability by Melting Peak Analysis with BAn6 and BAnS Specific an
BAT26: MSS
Tm: 51.2°C
TI -Nt
1'2 -N2
B
H 2 0
.o.Q4 ... ,
I
I
I
•
,
I
I
I
I
I
I
I
I
•
I t
I
I
I
I
43.0 42.5 -4G.D ~J!, GOoO 52.5 >56.0 67"" Il10.0 02.5 00:.0 1:11.0 10,0 n .o 70J) 77 .0 80.0 82Jl 80.0 17.0 1iJIO.u~
Tempemure rc)
BAT26: MSI-H
0.28 -
45.5°C
TI -...
Nl
T2 -N2
H 2 O
-0,04 -,
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
4).0 4:2.6 0lil50 476 000 02A 000 576 tIO.o 02.6 060 tI1A 10.0 nJ! 1150 n 6 10..0 82.5 MQ 87& 00..0 G'28
Temperature rc)
Fig. I. a BAT26 MSS. Melting peak analysis [-d(F2/Fl)/dTl of DNA from two representative MSS
tumors (Tl, T2), two normal mucosa samples (Nl, N2), and a control DNA from mononuclear
blood cells (B). b BAT26 MSI-H. Melting peak analysis [-d(F2/Fl)/dTl of DNA from two representative MSI-H tumors (Tl, T2) and two normal mucosa samples (Nl, N2)
'a
:::&.
...
~
l&V
• u c
I
J
"' "
~
~ ...
Yo
~
V
• u
8
• !
8
~
0.2tO~ -
020022 -
Analysis of Microsatellite Instability by Melting Peak Analysis with BAn6 and BAnS Specific an
BAT26: MSS
Tm: 51.2°C
TI -Nt
1'2 -N2
B
H 2 0
.o.Q4 ... ,
I
I
I
•
,
I
I
I
I
I
I
I
I
•
I t
I
I
I
I
43.0 42.5 -4G.D ~J!, GOoO 52.5 >56.0 67"" Il10.0 02.5 00:.0 1:11.0 10,0 n .o 70J) 77 .0 80.0 82Jl 80.0 17.0 1iJIO.u~
Tempemure rc)
BAT26: MSI-H
0.28 -
45.5°C
TI -...
Nl
T2 -N2
H 2 O
-0,04 -,
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
4).0 4:2.6 0lil50 476 000 02A 000 576 tIO.o 02.6 060 tI1A 10.0 nJ! 1150 n 6 10..0 82.5 MQ 87& 00..0 G'28
Temperature rc)
Fig. I. a BAT26 MSS. Melting peak analysis [-d(F2/Fl)/dTl of DNA from two representative MSS
tumors (Tl, T2), two normal mucosa samples (Nl, N2), and a control DNA from mononuclear
blood cells (B). b BAT26 MSI-H. Melting peak analysis [-d(F2/Fl)/dTl of DNA from two representative MSI-H tumors (Tl, T2) and two normal mucosa samples (Nl, N2)