Recovery of Proteins and Microorganisms from Cultivation Media by Foam Flotation
221
Fig. 6. Continuous cell flotation column. 1 magnetic stirrer; 2 cell suspension; 3 pump; 4 feed;
5 liquid outlet with cell residue; 6 thermostat; 7 foam breaker; and 8 foam liquid [113]
feed, the foam liquid and the residue liquid, respectively. In addition, the surface
tension s and foaminess S of the cultivation media were determined.
Hansenula polymorpha (CBS 4732) was cultivated in a synthetic nutrient
medium [113] with glucose, ethanol and methanol as substrates, respectively, in
batch and continuous operations. The flotation was performed in batch as well
as in continuous mode. A comparison of the performances of batch flotation
with continuous flotation indicated that, in the latter, the C S * values are higher
than in the former. For example, at C P * = 1.5 g l –1 , the cell concentration in the
221
Fig. 6. Continuous cell flotation column. 1 magnetic stirrer; 2 cell suspension; 3 pump; 4 feed;
5 liquid outlet with cell residue; 6 thermostat; 7 foam breaker; and 8 foam liquid [113]
feed, the foam liquid and the residue liquid, respectively. In addition, the surface
tension s and foaminess S of the cultivation media were determined.
Hansenula polymorpha (CBS 4732) was cultivated in a synthetic nutrient
medium [113] with glucose, ethanol and methanol as substrates, respectively, in
batch and continuous operations. The flotation was performed in batch as well
as in continuous mode. A comparison of the performances of batch flotation
with continuous flotation indicated that, in the latter, the C S * values are higher
than in the former. For example, at C P * = 1.5 g l –1 , the cell concentration in the