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G 151 K 152 , L153_K154 and the C terminal peptide T163_D165 (291.10) could not be identified as their mass values fell below the lower limit of data acquisition. All the
identified pep tides corresponded to total cleavage on the tryptic sites. Starting
with the glycosylated protein on a gel band, the entire process was accomplished
in one working day: in-gel deglycosylation (2 h), re-electrophoresis on a mini-gel
(1.8 h), detection (15 mins), gel crushing and digestion with trypsin (4.5 h) and
peptide extraction (30 mins).