Exploring Functions for Glycosylation in Host Defence
a
5
HPLC
normal IgG
HPLC
RA IgG
GOF
G2F
GII1.3)8
+
Glll,6)F
Retention time
6
I
112
9
116
10 gu
I
329
120 minutes
Fig. 23.6. Normal phase HPLC separation of total glycans from (a) pooled normal serum IgG and (b)
rheumatoid serum IgG. Abbreviations used for describing oligosaccharide structures: G(0-2) indicates the number of terminal galactose residues in the structure; F: fucose; B: bisecting N-acetyl glucosamine (GlcNAc); S: sialic acid
a
5
HPLC
normal IgG
HPLC
RA IgG
GOF
G2F
GII1.3)8
+
Glll,6)F
Retention time
6
I
112
9
116
10 gu
I
329
120 minutes
Fig. 23.6. Normal phase HPLC separation of total glycans from (a) pooled normal serum IgG and (b)
rheumatoid serum IgG. Abbreviations used for describing oligosaccharide structures: G(0-2) indicates the number of terminal galactose residues in the structure; F: fucose; B: bisecting N-acetyl glucosamine (GlcNAc); S: sialic acid