Enzymatic and Chemical Deblocking of N-Terminally Modified Proteins
319
References
Aitkin A (1990) Identification of protein consensus sequences, Ellis Horwood, Chichester, West Sussex
Gheorghe MT, Jornvall H, Bergman T (1997) Optimized alcoholytic deacetylation ofN-acetyl-blocked
polypeptides for subsequent Edman degradation. Anal Biochem 254: 119-125
Hirano H, Komatsu S, Nakamura A, Kikuchi F, Kajiwara H, Tsunasawa S, Sakiyama F (1991) Structural homology between semidwarfism-related proteins and glutelin seed protein in rice (Oryza
sativa L.,). Theor Appl Genet 83: 153-158
Hirano H (1997) Sequence analysis of the NH2-terminally blocked proteins immobilized on PVDF
membranes from polyacrylamide gels In: Kamp RM, Papadopoulou T, Wittmann-Liebold B (eds)
Protein Structure Analysis. Springer Verlag Berlin Heidelberg, pp 167-179
HuImes JD, Pan Y -C (1991) Selective cleavage of polypeptides with trifluoroacetic acid: Applications
for microsequencing. Anal Biochem 197: 368-376
Ikeuchi M, Inoue Y (1988) A new photosystem II reaction center component (4,8 kDa protein)
encoded by chloroplast genome. FEBS Lett 241: 99-lO4
Kamp RM, Tsunasawa S, Hirano H (1998) Application of new deblocking aminopeptidase from Pyrococcus furiosus for microsequence analysis of blocked proteins. J.Prot. Chern. 17: 512-513
Kraft R (1997) Enzymatic and chemical cleavages of proteins. In: Kamp RM, PapadopouIou T,
Wittmann-Liebold B (eds) Protein Structure Analysis. Springer Verlag Berlin Heidelberg, pp63-71
Krishna RG, Christopher CQ, Wold F (1991) N-terminal sequence analysis of N-acetylated proteins
after unblocking with N -acylaminacyl peptide hydrolase. Anal Biochem 199: 45 - 50
Mitsunaga K, Miyagi T, Ishimizu T, Kato I, Tsunasawa S (1993) A novel method for specific isolation
of the N-terminally blocked peptide from proteins. Proc. Jap. Prot. Symp.
Miyatake N, Kamo M, Satake K, Uchiyama Y, Tsugita A (1992) Removal ofN-terminal formyl groups
and deblocking of pyrrolidone carboxylic acid of proteins with anhydrous hydrazine vapor. Eur J
Biochem 212: 785-789
Takakura H, Tsunasawa S, Miyagi M, Warner J (1992) NH2-terminal acetylation of ribosomal protein
of Saccharomyces cerevisiae. J Bioi Chern 267: 5442-5445
Tsunasawa S (1998) Purification and application of a novel N-terminal deblocking aminopeptidase
(DAP) from Pyrococcus Furiosus. J Protein Chern 17: 521-5 22
Tsunasawa S, Hirano H (1993) Deblocking and subsequent microsequence analysis of N-terminally
blocked proteins immoblized on PVDF membrane, In: Imahori K, Sakiyama F (eds) Methods in
protein sequence analysis. Plenum, New York, pp 43-51
Tsunasawa S, Sakiyama F (1992) Amino-terminal acetylation of proteins. In: Tuboi S, Taniguchi N,
Katsumara N (eds) The posttranslational modification of proteins. Japan Scientific Societies,
Tokyo, pp 113-121
Tsunasawa S, Takakura H, Sakiyama F (1990) Microsequence analysis of N -acetylated proteins. J Protein Chern: 265-266
Wellner D, Panneerselvam C, Horecker BL (1990) Sequencing of peptides and proteins with blocked
N-terminal amino acids: N-acetylserine or N-acetylthreonine. Proc Nat! Acad Sci USA 87:
1947 -1949
319
References
Aitkin A (1990) Identification of protein consensus sequences, Ellis Horwood, Chichester, West Sussex
Gheorghe MT, Jornvall H, Bergman T (1997) Optimized alcoholytic deacetylation ofN-acetyl-blocked
polypeptides for subsequent Edman degradation. Anal Biochem 254: 119-125
Hirano H, Komatsu S, Nakamura A, Kikuchi F, Kajiwara H, Tsunasawa S, Sakiyama F (1991) Structural homology between semidwarfism-related proteins and glutelin seed protein in rice (Oryza
sativa L.,). Theor Appl Genet 83: 153-158
Hirano H (1997) Sequence analysis of the NH2-terminally blocked proteins immobilized on PVDF
membranes from polyacrylamide gels In: Kamp RM, Papadopoulou T, Wittmann-Liebold B (eds)
Protein Structure Analysis. Springer Verlag Berlin Heidelberg, pp 167-179
HuImes JD, Pan Y -C (1991) Selective cleavage of polypeptides with trifluoroacetic acid: Applications
for microsequencing. Anal Biochem 197: 368-376
Ikeuchi M, Inoue Y (1988) A new photosystem II reaction center component (4,8 kDa protein)
encoded by chloroplast genome. FEBS Lett 241: 99-lO4
Kamp RM, Tsunasawa S, Hirano H (1998) Application of new deblocking aminopeptidase from Pyrococcus furiosus for microsequence analysis of blocked proteins. J.Prot. Chern. 17: 512-513
Kraft R (1997) Enzymatic and chemical cleavages of proteins. In: Kamp RM, PapadopouIou T,
Wittmann-Liebold B (eds) Protein Structure Analysis. Springer Verlag Berlin Heidelberg, pp63-71
Krishna RG, Christopher CQ, Wold F (1991) N-terminal sequence analysis of N-acetylated proteins
after unblocking with N -acylaminacyl peptide hydrolase. Anal Biochem 199: 45 - 50
Mitsunaga K, Miyagi T, Ishimizu T, Kato I, Tsunasawa S (1993) A novel method for specific isolation
of the N-terminally blocked peptide from proteins. Proc. Jap. Prot. Symp.
Miyatake N, Kamo M, Satake K, Uchiyama Y, Tsugita A (1992) Removal ofN-terminal formyl groups
and deblocking of pyrrolidone carboxylic acid of proteins with anhydrous hydrazine vapor. Eur J
Biochem 212: 785-789
Takakura H, Tsunasawa S, Miyagi M, Warner J (1992) NH2-terminal acetylation of ribosomal protein
of Saccharomyces cerevisiae. J Bioi Chern 267: 5442-5445
Tsunasawa S (1998) Purification and application of a novel N-terminal deblocking aminopeptidase
(DAP) from Pyrococcus Furiosus. J Protein Chern 17: 521-5 22
Tsunasawa S, Hirano H (1993) Deblocking and subsequent microsequence analysis of N-terminally
blocked proteins immoblized on PVDF membrane, In: Imahori K, Sakiyama F (eds) Methods in
protein sequence analysis. Plenum, New York, pp 43-51
Tsunasawa S, Sakiyama F (1992) Amino-terminal acetylation of proteins. In: Tuboi S, Taniguchi N,
Katsumara N (eds) The posttranslational modification of proteins. Japan Scientific Societies,
Tokyo, pp 113-121
Tsunasawa S, Takakura H, Sakiyama F (1990) Microsequence analysis of N -acetylated proteins. J Protein Chern: 265-266
Wellner D, Panneerselvam C, Horecker BL (1990) Sequencing of peptides and proteins with blocked
N-terminal amino acids: N-acetylserine or N-acetylthreonine. Proc Nat! Acad Sci USA 87:
1947 -1949