318
1
Lysylendopeptidase
1
Carboxypeptidase B
Peptide
+ NH2~ + Lys
1
DITC - Glass
Roo Moo KAMP and Hoo HIRANO
Peptide
+NH2~
Peptide
Peptide
Blocked - Aj - A2- A3 +
/"... +
~./
NH{" 'NH{ .. ........,..,..
+
Lys
\
1
Biotin
Peptide
Peptide
B o o • ~ +
/ ' 0 . : . /
IOtm
Biotin' ........,..,..
1
Avidin column
1
Sequencing
Mass spectrometry
DITC - Glass
Fig. 22.8. Isolation of the N-terminal peptide from N-terminally blocked proteins (from Mitsunaga
et al. 1993)