312
R. M. KAMP and H. HIRANO
molecular mass (Tsunasawa et al. 1990). In this case, prior to AARE digestion,
proteins in the solutions or on the membrane should be digested with proteases
such as trypsin. When the treatment is performed, non blocked peptides and Nacetylated peptides are detected. The resulting pep tides can be easily removed
Peptide
NH2~COOH
! Q-N=C=S
~O~
Peptide
NH-IT-NH~COOH
S
! Ox,datio,
! AARE
!
Sequencing
A2- A3
PTC - Peptide
PC - Peptide
PC - Peptide
Fig. 22.5. Deblocking ofN-acetylated proteins with acylamino acid releasing enzyme (AARE). (From
Tsunasawa et al. 1990)
R. M. KAMP and H. HIRANO
molecular mass (Tsunasawa et al. 1990). In this case, prior to AARE digestion,
proteins in the solutions or on the membrane should be digested with proteases
such as trypsin. When the treatment is performed, non blocked peptides and Nacetylated peptides are detected. The resulting pep tides can be easily removed
Peptide
NH2~COOH
! Q-N=C=S
~O~
Peptide
NH-IT-NH~COOH
S
! Ox,datio,
! AARE
!
Sequencing
A2- A3
PTC - Peptide
PC - Peptide
PC - Peptide
Fig. 22.5. Deblocking ofN-acetylated proteins with acylamino acid releasing enzyme (AARE). (From
Tsunasawa et al. 1990)