278
Fig. 20.4. SDS-PAGE (15 %-discontinuous
system) of the purified TthS14. 5 flg and
10 flg of protein were loaded on the gel. M:
molecular weight markers. Staining of the
gel was performed with Coomassie blue
Fig. 20.5. SDS-PAGE (15 %-discontinuous
system) of pure TthS14. Lane 1: 5 flg of
pure TthS 14 without heating and in the
absence of ~-mercaptoethanol. Lane 2: 5 flg
of pure TthS 14 after heating at 100°C for
5 mins and in the presence of 5 mM
~-mercaptoethanol
kDa
43
29
18
14
6
3
43
29
18
14
6
3
P. TSIBOLI et al.
M
1
2
M 1 2