VIII
Contents
Subprotocol 3: Protocol for Co denaturation ............ 90
Subprotocol4: Comparative Genomic Hybridization (CGH) 92
Chapter 7
In Situ Hybridization for RNA: Radioactive DNA Probe
T. SA WAI, M. UZUKI ..................•...........•.. 100
Chapter 8
In Situ Hybridization for RNA: Radioactive RNA
Probe - A Protocol for Practice With a Kit
H. YOSHIOKA, S. NOJI . . . . . . . . . . . . . . . . . . . . . . . . . • . . . . .. 115
Chapter 9
In Situ Hybridization for RNA: Nonradioactive probe:
ds cDNA Probe
H. MATSUMI, T. YANO
Chapter 10
In Situ Hybridization for RNA: Nonradioactive Probe:
ss cDNA Probe
Y. KANEMITSU, T. KOJI
Chapter 11
In Situ Hybridization for RNA: Nonradioactive Probe:
Oligo-DNA Probe: T-T Dimer
Y. HISHIKA W A, N. NAGASUE, M. MURAKAMI,
128
139
M. KASAHARA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 154
Chapter 12
In Situ Hybridization for RNA: Nonradioactive Probe:
Oligo-DNA Probe: Digoxigenin (I)
Y. IWASAKI, 1. KATO ........................•........ 172
Chapter 13
In Situ Hybridization for RNA: Nonradioactive Probe:
Oligo-DNA Probe: Digoxigenin (II)
M. MIYAZAKI, Y. OZONO, T. HARADA, S. KOHNO . . . . . . .. 182
Chapter 14
In Situ Hybridization for RNA: Nonradioactive Probe:
RNA Probe
H.OHTANI ......................................... 196