Contents
Part I
Chapter 1
Introduction to the Detection of DNA Strand Breaks
T. KOJI .............................................
3
Chapter 2
In Situ Nick Translation
S. HASHIMOTO, T. KANEMATSU ...... '" . . . . . .. . .... .. 13
Chapter 3
In Situ Nick End-Labeling: Light Microscopical
s. OBA, T. KOJI ...................................... 19
Chapter 4
In Situ Nick End-Labeling: Electron Microscopical
Y. OTSUKI, Y. ITO ................................... 31
Part II
Chapter 5
Introduction to the Detection of Specific DNA
and RNA Sequences
T. KOJI ............................................. 49
Chapter 6
In Situ Hybridization for DNA: Fluorescent Probe
M. MATSUT A, M. MATSUT A .............. . . . . . . . . . . . .. 65
Subprotocol 1: Fluorescence In Situ Hybridization (FISH) 68
Subprotocol 2: Protocol for Rapid Procedure ........... 88