38
YOSHINORI OTSUKI AND YUKO ITO
Fig. 4. Electron micrograph stained with the ISELITEM technique (X 15,000).
The nuclei (arrows) of apoptotic endometrial glandular cell at the late secretory
phase is characterized by the presence of condensed chromatin with many immunogold particles. Note the presence of many immunogolds (shown in black
spots) only at the condensed chromatin but not at the other nuclear chromatin.
ISEL technique involving immunoperoxidase method is unsuitable for the study of the epidermis and eosinophils.
The target of the ISEL/TEM technique is the newly formed 3' -OH
DNA ends. A few immunogold particles indicating 3'-OH DNA
ends are observed on the evenly distributed chromatin of cells
undergoing proliferation, although the conventional ISEL technique does not stain the proliferous cells. This finding may be
due to an artifact from oxidant treatment, section cutting (Ansari et al. 1993), DNA breaks induced by DNA repair systems or
sites of active gene transcription located in the chromatin (Thiry
1991), rather than oligonucleosomal degradation.
• The labeling is certainly conditioned by the concentration
and incubation time of oxidants for etching. The application
of saturated sodium metaperiodate for only 2 mins yields
YOSHINORI OTSUKI AND YUKO ITO
Fig. 4. Electron micrograph stained with the ISELITEM technique (X 15,000).
The nuclei (arrows) of apoptotic endometrial glandular cell at the late secretory
phase is characterized by the presence of condensed chromatin with many immunogold particles. Note the presence of many immunogolds (shown in black
spots) only at the condensed chromatin but not at the other nuclear chromatin.
ISEL technique involving immunoperoxidase method is unsuitable for the study of the epidermis and eosinophils.
The target of the ISEL/TEM technique is the newly formed 3' -OH
DNA ends. A few immunogold particles indicating 3'-OH DNA
ends are observed on the evenly distributed chromatin of cells
undergoing proliferation, although the conventional ISEL technique does not stain the proliferous cells. This finding may be
due to an artifact from oxidant treatment, section cutting (Ansari et al. 1993), DNA breaks induced by DNA repair systems or
sites of active gene transcription located in the chromatin (Thiry
1991), rather than oligonucleosomal degradation.
• The labeling is certainly conditioned by the concentration
and incubation time of oxidants for etching. The application
of saturated sodium metaperiodate for only 2 mins yields