12 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe
181
trol probe using an image analyzer (lEAS 2000 system, Carl
Zeiss).
References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction
product. J Histochem Cytochem 29: 775
Denjin M, De Weger RA, Berends MJH, Compier-Spies PI, Jansz H, Van
Unnik JAM, Lips CJM (1990) Detection of calcitonin-encoding mRNA
by radioactive and non-radioactive in situ hybridization: improved colorimetric detection and cellular localization of mRNA in thyroid sections. J Histochem Cytochem 38: 351-358
Iwasaki Y, Hara Y, Koji T, Shibata Y, Nakane PK, Kato I (1998) Differential
expression of IFN -y,IL-4, IL-IO, and IL-l ~ mRNAs in decalcified tissue
sections of mouse lipopolysaccharide-induced periodontitis mandibles
assessed by in situ hybridization. Histochem and Cell BioI 1090:339-347
Koji T, Brenner RM (1993) Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132: 382-392
Koji T, Nakane PK (1990) Localization in situ of specific mRNA using thymine-thymine dimmerized DNA probes. Sensitive and reliable nonradioactive in situ hybridization. Acta Pathol Jpn 40: 793-807
Koji T, N akane PK (1996) Recent advances in molecular histochemical techniques: in situ hybridization and southwestern histochemistry. J Electron
Microsc 45: 119-127
Koji T, Chedid M, Rubin JS, Slayden aD, Csaky KG, Aaronson SA, Brenner
RM (1994) Progesterone-dependent expression of keratinocyte growth
factor mRNA in stromal cells of the primate endometrium: keratinocyte
growth factor as a progestomedin. J Cell BioI 125: 393-401
Koji T, Moriuchi T, Nakane PK (1988) Improved tissue preparation for in
situ localization of specific mRNA using non-radioactive DNA probes:
effects of protease digestion and probe size on signal detection in frozen
and paraffin sections of rat pituitary glands. Acta Histochem Cytochem
21: 187-200
Koji T, Sugawara I, Kimura M, Nakane PK (1989) In situ localization of
c-myc mRNA in HL-60 cells using non-radioactive synthetic oligodeoxynucleotide probes. Acta Histochem Cytochem 22: 295-307
Unger ER, Hammer ML, Chenggis ML (1991) Comparison of 35 S and biotin
as labels for in situ hybridization: use of an HPV model system. J Histo chern Cytochem 39: 145-150
Yoshii A, Koji T, Ohsawa N, Nakane PK (1995) In situ localization of ribosomal RNAs is a reliable reference for hybridizable RNA in tissue sections. J Histochem Cytochem 43: 321-327
181
trol probe using an image analyzer (lEAS 2000 system, Carl
Zeiss).
References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction
product. J Histochem Cytochem 29: 775
Denjin M, De Weger RA, Berends MJH, Compier-Spies PI, Jansz H, Van
Unnik JAM, Lips CJM (1990) Detection of calcitonin-encoding mRNA
by radioactive and non-radioactive in situ hybridization: improved colorimetric detection and cellular localization of mRNA in thyroid sections. J Histochem Cytochem 38: 351-358
Iwasaki Y, Hara Y, Koji T, Shibata Y, Nakane PK, Kato I (1998) Differential
expression of IFN -y,IL-4, IL-IO, and IL-l ~ mRNAs in decalcified tissue
sections of mouse lipopolysaccharide-induced periodontitis mandibles
assessed by in situ hybridization. Histochem and Cell BioI 1090:339-347
Koji T, Brenner RM (1993) Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132: 382-392
Koji T, Nakane PK (1990) Localization in situ of specific mRNA using thymine-thymine dimmerized DNA probes. Sensitive and reliable nonradioactive in situ hybridization. Acta Pathol Jpn 40: 793-807
Koji T, N akane PK (1996) Recent advances in molecular histochemical techniques: in situ hybridization and southwestern histochemistry. J Electron
Microsc 45: 119-127
Koji T, Chedid M, Rubin JS, Slayden aD, Csaky KG, Aaronson SA, Brenner
RM (1994) Progesterone-dependent expression of keratinocyte growth
factor mRNA in stromal cells of the primate endometrium: keratinocyte
growth factor as a progestomedin. J Cell BioI 125: 393-401
Koji T, Moriuchi T, Nakane PK (1988) Improved tissue preparation for in
situ localization of specific mRNA using non-radioactive DNA probes:
effects of protease digestion and probe size on signal detection in frozen
and paraffin sections of rat pituitary glands. Acta Histochem Cytochem
21: 187-200
Koji T, Sugawara I, Kimura M, Nakane PK (1989) In situ localization of
c-myc mRNA in HL-60 cells using non-radioactive synthetic oligodeoxynucleotide probes. Acta Histochem Cytochem 22: 295-307
Unger ER, Hammer ML, Chenggis ML (1991) Comparison of 35 S and biotin
as labels for in situ hybridization: use of an HPV model system. J Histo chern Cytochem 39: 145-150
Yoshii A, Koji T, Ohsawa N, Nakane PK (1995) In situ localization of ribosomal RNAs is a reliable reference for hybridizable RNA in tissue sections. J Histochem Cytochem 43: 321-327