114
TAKASHI SAWAI AND MIWA UZUKI
• Culture cells
Fig. 3 shows the grains relevant to MMP-l expressed on the
culture cells of synoviocytes from rheumatoid arthritis. The
left side of Fig. 3 shows the synoviocyte expressing mRNA stimulated by Interleukin-l ( IL-1) , while as shown on the right
side, no silver grains were detected in the cells stimulated by
Interferon-y (IFN-y).
• Quantity of mRNA in ISH
The number of grains is countable, which is available for the
comparison of mRNA volumes. Fig. 4 shows the number of
MMP-3 mRNA positive cells comprising rheumatoid synovia.
Many grains were massively demonstrated in the superficial
cells of synovial tissues.
References
Brady MAW and Finlan M (1990) Radioactive labels: autoradiography and
choice of emulsions for in situ hybridization. In In situ hybridization -
principles and practice- (eds. JM Polak and JO' D McGEE), Oxford University Press Oxford - New York - Tokyo
Hafen E, Levine M, Garber LG and Gehring WJ (l983) An improved in stiu
hybridization method for the detection of cellular RNAs in Drosophilia
tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex. The EMBO J 2:617-23
Hayashi M, Ninomiya Y, Parson J et al (l986) Differential localization of
mRNAs of collagen types I and II in chick fibroblasts, chondrocytes,
and corneal cells by in situ hybridization using cDNA probes. J Cell
BioI 102: 2302-9
Pardue ML and Gall JG (l969) Molecular hybridization of radioactive DNA
to the DNA of cytological preparations. Proc. Natl Acad Sci 70: 1860-4
Sawai T and Uzuki M (1996) In situ hybridization by radioactive cDNA
probes - practice and application- In Histochemistry i n 1996. ed by
K Nakane, PP129-136 ,Gakusai Kikaku, Tokyo
Sawai T, Uzuki M, Harris Jr. ED, Kurkinnen M, Trelstad RL and Hayashi M
(l996) In situ hybridization of stromelysin mRNA in the synovial biopsies from rheumatoid arthritis. Tohoku J. Exp. Med. 178: 315 - 330
TAKASHI SAWAI AND MIWA UZUKI
• Culture cells
Fig. 3 shows the grains relevant to MMP-l expressed on the
culture cells of synoviocytes from rheumatoid arthritis. The
left side of Fig. 3 shows the synoviocyte expressing mRNA stimulated by Interleukin-l ( IL-1) , while as shown on the right
side, no silver grains were detected in the cells stimulated by
Interferon-y (IFN-y).
• Quantity of mRNA in ISH
The number of grains is countable, which is available for the
comparison of mRNA volumes. Fig. 4 shows the number of
MMP-3 mRNA positive cells comprising rheumatoid synovia.
Many grains were massively demonstrated in the superficial
cells of synovial tissues.
References
Brady MAW and Finlan M (1990) Radioactive labels: autoradiography and
choice of emulsions for in situ hybridization. In In situ hybridization -
principles and practice- (eds. JM Polak and JO' D McGEE), Oxford University Press Oxford - New York - Tokyo
Hafen E, Levine M, Garber LG and Gehring WJ (l983) An improved in stiu
hybridization method for the detection of cellular RNAs in Drosophilia
tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex. The EMBO J 2:617-23
Hayashi M, Ninomiya Y, Parson J et al (l986) Differential localization of
mRNAs of collagen types I and II in chick fibroblasts, chondrocytes,
and corneal cells by in situ hybridization using cDNA probes. J Cell
BioI 102: 2302-9
Pardue ML and Gall JG (l969) Molecular hybridization of radioactive DNA
to the DNA of cytological preparations. Proc. Natl Acad Sci 70: 1860-4
Sawai T and Uzuki M (1996) In situ hybridization by radioactive cDNA
probes - practice and application- In Histochemistry i n 1996. ed by
K Nakane, PP129-136 ,Gakusai Kikaku, Tokyo
Sawai T, Uzuki M, Harris Jr. ED, Kurkinnen M, Trelstad RL and Hayashi M
(l996) In situ hybridization of stromelysin mRNA in the synovial biopsies from rheumatoid arthritis. Tohoku J. Exp. Med. 178: 315 - 330