7 In Situ Hybridization for RNA: Radioactive DNA Probe
III
Results
• mRNA associated with production of specific amino acid
could be detected by expression of silver grains. Control studies are necessary for confirming the positive results using
well-known probes, RNase treatment and other negative tissues or cells.
• Immunohistochemical (IHe) study should be performed, if
possible, and compared with the result of in situ hybridization
to obtain more information about the positive cells.
• Northern blot is also an advantage for obtaining information
as to whether or not the tissues used contain enough mRNA.
• IHC and hematoxylin-eosin stain reveals information about
the cells and tissues relevant to cellular dynamics, if effectively
used with ISH
• RI-ISH, whether or not mRNA is expressed, shows activity
and dynamics of the cells and tissues in morphogenesis
" .
Fig. 1. mRNA of Type II collagen in the limb of chick embryo at stage 28.
28
III
Results
• mRNA associated with production of specific amino acid
could be detected by expression of silver grains. Control studies are necessary for confirming the positive results using
well-known probes, RNase treatment and other negative tissues or cells.
• Immunohistochemical (IHe) study should be performed, if
possible, and compared with the result of in situ hybridization
to obtain more information about the positive cells.
• Northern blot is also an advantage for obtaining information
as to whether or not the tissues used contain enough mRNA.
• IHC and hematoxylin-eosin stain reveals information about
the cells and tissues relevant to cellular dynamics, if effectively
used with ISH
• RI-ISH, whether or not mRNA is expressed, shows activity
and dynamics of the cells and tissues in morphogenesis
" .
Fig. 1. mRNA of Type II collagen in the limb of chick embryo at stage 28.
28