226
P. ARESE, A. BOSIA, and L. ROSSINI
3. BRADY, A.].: In: Physiology of the Amphibia (A.]. MOORE, Ed.). New
York-London: Academic Press 1965.
4. BUCHER, TH., U. W. RUSSMANN: Angew. Chern. 75, 881 (1963).
5. DIXON, W.: Biomedical Computer Programs, Dept. of Preventive Medicine
and Public Health. Los Angeles: University of California Medical School
1964.
6. DRUMMOND, G. 1., and L. DUNCAN: BioI. Chern. 241,3097 (1966).
7. GARFINKEL, D.: In: Instrumentation in Biochemistry, (T. W. GOODWIN, Ed.)
p. 81, Biochem. Soc. Symp. Nr. 26. New York-London: Academic Press
1967.
8. HIGGINS, ].].: In: Computers in Biochemical Research (R. W. STACY and
B. WAXMAN, Eds.), Vol. 2, p. 101. New York-London: Academic Press
1965.
9. HOHORST, H. ].: Habilitationsschrift. MarburgjLahn 1962.
10. - In: Funktionelle und morphologische Organisation der Zelle (P. KARLSON, Ed.), p. 163. Berlin-Gottingen-Heidelberg: Springer-Verlag 1963.
11. MARGRETH, A., C. CATANI and S. SCHIAFFINO: Biochem.]. 102, 35C (1967).
12. WILLIAMSON,]. R.: ]. BioI. Chern. 240,2308 (1965).
13. - ]. BioI. Chern., 241, 5026 (1966).
14. WOLLENBERGER, A.: Arch. Exp. Path. Pharm. 219, 408 (1953).
Aknowledgement
This work was supported by the Italian Research Council (Consiglio Nazionale
delle Ricerche), Rome.
Discussion
WALTER:
In the interconversion of F-6-P and FDP phosphofructokinase catalyzes the
reaction in one direction and FDP phosphatase catalyzes the reverse reaction.
Have you any idea, perhaps from tracer experiments, whether the source of
F-6-P buildup is G-6-P or FDP?
ARESE:
The presence of FDP phosphatase in skeletal muscle is well established
(M. SALAS, E. VINUELA, ]. SALAS and A. SOLS, Biochem. Biophys. Res. Comm.
17, 150 (1964)). However, till now, no evidence of this enzyme in heart tissue
of several species has been found (H. A. KREBS and M. WOODFORD, Biochem.
]. 94 436 (1965)). We think therefore that the observed effects are caused by
the interaction of ions or of regulative metabolites with phosphofructokinase.
P. ARESE, A. BOSIA, and L. ROSSINI
3. BRADY, A.].: In: Physiology of the Amphibia (A.]. MOORE, Ed.). New
York-London: Academic Press 1965.
4. BUCHER, TH., U. W. RUSSMANN: Angew. Chern. 75, 881 (1963).
5. DIXON, W.: Biomedical Computer Programs, Dept. of Preventive Medicine
and Public Health. Los Angeles: University of California Medical School
1964.
6. DRUMMOND, G. 1., and L. DUNCAN: BioI. Chern. 241,3097 (1966).
7. GARFINKEL, D.: In: Instrumentation in Biochemistry, (T. W. GOODWIN, Ed.)
p. 81, Biochem. Soc. Symp. Nr. 26. New York-London: Academic Press
1967.
8. HIGGINS, ].].: In: Computers in Biochemical Research (R. W. STACY and
B. WAXMAN, Eds.), Vol. 2, p. 101. New York-London: Academic Press
1965.
9. HOHORST, H. ].: Habilitationsschrift. MarburgjLahn 1962.
10. - In: Funktionelle und morphologische Organisation der Zelle (P. KARLSON, Ed.), p. 163. Berlin-Gottingen-Heidelberg: Springer-Verlag 1963.
11. MARGRETH, A., C. CATANI and S. SCHIAFFINO: Biochem.]. 102, 35C (1967).
12. WILLIAMSON,]. R.: ]. BioI. Chern. 240,2308 (1965).
13. - ]. BioI. Chern., 241, 5026 (1966).
14. WOLLENBERGER, A.: Arch. Exp. Path. Pharm. 219, 408 (1953).
Aknowledgement
This work was supported by the Italian Research Council (Consiglio Nazionale
delle Ricerche), Rome.
Discussion
WALTER:
In the interconversion of F-6-P and FDP phosphofructokinase catalyzes the
reaction in one direction and FDP phosphatase catalyzes the reverse reaction.
Have you any idea, perhaps from tracer experiments, whether the source of
F-6-P buildup is G-6-P or FDP?
ARESE:
The presence of FDP phosphatase in skeletal muscle is well established
(M. SALAS, E. VINUELA, ]. SALAS and A. SOLS, Biochem. Biophys. Res. Comm.
17, 150 (1964)). However, till now, no evidence of this enzyme in heart tissue
of several species has been found (H. A. KREBS and M. WOODFORD, Biochem.
]. 94 436 (1965)). We think therefore that the observed effects are caused by
the interaction of ions or of regulative metabolites with phosphofructokinase.